An important technological revolution is underway in the field of neuroscience as we begin
the 21st century. The combination of optical methods with genetically encoded …

Two-photon excitation with temporally focused pulses can be combined with phase-
modulation approaches, such as computer-generated holography and generalized phase …

Optical means for modulating and monitoring neuronal activity, have provided substantial
insights to neurophysiology and toward our understanding of how the brain works …

Two-photon voltage imaging has long been heralded as a transformative approach capable
of answering many long-standing questions in modern neuroscience. However, exploiting …

A technical challenge in neuroscience is to record and specifically manipulate the activity of
neurons in living animals. This can be achieved in some preparations with two-photon …

Significance: Light-field microscopy (LFM) enables high signal-to-noise ratio (SNR) and light
efficient volume imaging at fast frame rates. Voltage imaging with genetically encoded …

Computer-generated holography enables efficient light pattern generation through phase-
only wavefront modulation. While perfect patterning usually requires control over both phase …

Recent years have seen substantial developments in technology for imaging neural circuits,
raising the prospect of large-scale imaging studies of neural populations involved in …

Optogenetic approaches combine the power to allocate optogenetic tools (proteins) to
specific cell populations (defined genetically or functionally) and the use of light-based …

Light-field microscopy (LFM) is a type of all-optical imaging system that is able to capture 4D
geometric information of light rays and can reconstruct a 3D model from a single snapshot …