Fluorescence microscopy images should not be treated as perfect representations of
biology. Many factors within the biospecimen itself can drastically affect quantitative …

Live-cell fluorescence spectral imaging is an evolving modality of microscopy that uses
specific properties of fluorophores, such as excitation or emission spectra, to detect multiple …

Motivation Multispectral biological fluorescence microscopy has enabled the identification of
multiple targets in complex samples. The accuracy in the unmixing result degrades (i) as the …

Hyperspectral imaging (HSI) technology has been applied in a range of fields for target
detection and mixture analysis. While HSI was originally developed for remote sensing …

Background Flow cytometry is a powerful tool for identifying and quantifying various cell
markers, such as viability, vitality, and individual cell age, at single‐cell stages. However …

Spectral flow cytometry is a new technology that enables measurements of fluorescent
spectra and light scattering properties in diverse cellular populations with high precision …

The accuracy of assigning fluorophore identity and abundance, known as spectral unmixing,
in biological fluorescence microscopy images remains a significant challenge due to the …

Motivation Spectral imaging is often used to observe different objects with multiple
fluorescent labels to reveal the development of the biological event. As the number of …

The importance of mechanical force in biology is evident across diverse length scales,
ranging from tissue morphogenesis during embryo development to mechanotransduction …

Three-dimensional microscopic approaches in histopathology display multiplex properties
that present puzzling questions for specimens as related to their comprehensive volumetric …