[PDF][PDF] A threshold selection method from gray-level histograms

N Otsu - Automatica, 1975 - dspace.tul.cz
Summary 16S rRNA-targeted oligonucleotide probes for eubacteria (EUB338), ammonium-
oxidizing bacteria (Nsm156) and nitrite-oxidizing bacteria (Nb1000) were used for the rapid …

Two-photon fluorescence excitation and related techniques in biological microscopy

A Diaspro, G Chirico, M Collini - Quarterly reviews of biophysics, 2005 - cambridge.org
1. Introduction 982. Historical background of two-photon effects 992.1 2PE 1002.2 Harmonic
generation 1002.3 Fluorescence correlation spectroscopy 1003. Basic principles of two …

Photobleaching

A Diaspro, G Chirico, C Usai, P Ramoino… - Handbook of biological …, 2006 - Springer
Thanks to the wide variety of applications, fluorescence microscopy is now one of the most
popular imaging techniques in biology (Weber, 1960; Lakowicz, 1999; Periasamy, 2001; …

A study on the antibacterial activity of one-dimensional ZnO nanowire arrays: effects of the orientation and plane surface

X Wang, F Yang, W Yang, X Yang - Chemical Communications, 2007 - pubs.rsc.org
In this study, ZnO nanowire arrays with different orientations were prepared. Confocal laser
scanning microscopy (CLSM) and field-emission scanning electron microscope (FE-SEM) …

An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy

C Dunsby, PMP Lanigan, J McGinty… - Journal of Physics D …, 2004 - iopscience.iop.org
Fluorescence imaging is used widely in microscopy and macroscopic imaging applications
for fields ranging from biomedicine to materials science. A critical component for any …

Multifocal multiphoton excitation and time correlated single photon counting detection for 3-D fluorescence lifetime imaging

S Kumar, C Dunsby, PAAD Beule, DM Owen… - Optics …, 2007 - opg.optica.org
We report a multifocal multiphoton time-correlated single photon counting (TCSPC)
fluorescence lifetime imaging (FLIM) microscope system that uses a 16 channel multi-anode …

Accepting from the best donor; analysis of long-lifetime donor fluorescent protein pairings to optimise dynamic FLIM-based FRET experiments

KJ Martin, EJ McGhee, JP Schwarz, M Drysdale… - Plos one, 2018 - journals.plos.org
FRET biosensors have proven very useful tools for studying the activation of specific
signalling pathways in living cells. Most biosensors designed to date have been predicated …

Two-versus one photon excitation laser scanning microscopy: critical importance of excitation wavelength

PG Bush, DL Wokosin, AC Hall - Frontiers in bioscience: a …, 2007 - pmc.ncbi.nlm.nih.gov
It is often anticipated that two-photon excitation (TPE) laser scanning microscopy should
improve cell survival and tissue penetration relative to conventional one-photon excitation …

Application of multiple parallel perfused microbioreactors and three-dimensional stem cell culture for toxicity testing

ZF Cui, X Xu, N Trainor, JT Triffitt, JPG Urban… - Toxicology in Vitro, 2007 - Elsevier
In this study, a multiple parallel perfused microbioreactor platform, TissueFlex®, was
developed which can be used to perform cell and tissue culture under almost uniform and …

[HTML][HTML] Multiphoton bleaching of red fluorescent proteins and the ways to reduce it

M Drobizhev, RS Molina, J Franklin - International journal of molecular …, 2022 - mdpi.com
Red fluorescent proteins and biosensors built upon them are potentially beneficial for two-
photon laser microscopy (TPLM) because they can image deeper layers of tissue, compared …