Circular dichroism (CD) is being increasingly recognised as a valuable technique for
examining the structure of proteins in solution. However, the value of many studies using CD …

The use of the methylotrophic yeast, Pichia pastoris, as a cellular host for the expression of
recombinant proteins has become increasing popular in recent times. P. pastoris is easier to …

The expression of heterologous proteins in microorganisms using genetic recombination is
still the high point in the development and exploitation of modern biotechnology. People can …

In this study α-lactalbumin was converted from the regular, native state to a folding variant
with altered biological function. The folding variant was shown to induce apoptosis in tumor …

The consumption of animal proteins in general, and dairy proteins in particular, is associated
with sustainability and animal welfare issues. Recombinant synthesis of milk proteins is …

α-Lactalbumin (α-LA) is a small (Mr 14,200), acidic (pI 4–5), Ca2+-binding protein. α-LA is a
regulatory component of lactose synthase enzyme system functioning in the lactating …

The methylotrophic yeast Pichia pastoris is an established protein expression host for the
production of industrial enzymes. This yeast can be grown to very high cell densities and …

Food is essential to provide energy for human cellular metabolism, and is usually made from
plants or animals. Beside plants and animals, other important food sources are made by …

Direct measurement of the thermodynamics of biomolecular interactions is now relatively
easy. Interpretation of these thermodynamics in simple molecular terms is not. Recent work …

Background The solubility of recombinant proteins expressed in bacteria is often
disappointingly low. Several strategies have been developed to improve the yield and one …