Towards efficient enzymatic saccharification of pretreated lignocellulose: enzyme inhibition by lignin-derived phenolics and recent trends in mitigation strategies

R Zhai, J Hu, M ** - Biotechnology Advances, 2022 - Elsevier
Lignocellulosic biorefinery based on sugar-platform has been considered as an efficient
strategy to replace fossil fuel-based refinery. In the bioconversion process, pretreatment is …

Lytic polysaccharide monooxygenases: enzymes for controlled and site-specific Fenton-like chemistry

B Bissaro, VGH Eijsink - Essays in biochemistry, 2023 - portlandpress.com
The discovery of oxidative cleavage of glycosidic bonds by enzymes currently known as lytic
polysaccharide monooxygenases (LPMOs) has profoundly changed our current …

A conserved second sphere residue tunes copper site reactivity in lytic polysaccharide monooxygenases

KR Hall, C Joseph, I Ayuso-Fernández… - Journal of the …, 2023 - ACS Publications
Lytic polysaccharide monooxygenases (LPMOs) are powerful monocopper enzymes that
can activate strong C–H bonds through a mechanism that remains largely unknown. Herein …

The “life-span” of lytic polysaccharide monooxygenases (LPMOs) correlates to the number of turnovers in the reductant peroxidase reaction

S Kuusk, VGH Eijsink, P Väljamäe - Journal of Biological Chemistry, 2023 - ASBMB
Lytic polysaccharide monooxygenases (LPMOs) are monocopper enzymes that degrade the
insoluble crystalline polysaccharides cellulose and chitin. Besides the H 2 O 2 cosubstrate …

Enhanced in situ H2O2 production explains synergy between an LPMO with a cellulose-binding domain and a single-domain LPMO

AA Stepnov, VGH Eijsink, Z Forsberg - Scientific Reports, 2022 - nature.com
Lytic polysaccharide monooxygenases (LPMOs) are mono-copper enzymes that catalyze
oxidative depolymerization of recalcitrant substrates such as chitin or cellulose. Recent work …

Electrochemical monitoring of heterogeneous peroxygenase reactions unravels LPMO kinetics

L Schwaiger, F Csarman, H Chang, O Golten… - ACS …, 2024 - ACS Publications
Biological conversion of plant biomass depends on peroxygenases and peroxidases acting
on insoluble polysaccharides and lignin. Among these are cellulose-and hemicellulose …

On the impact of carbohydrate-binding modules (CBMs) in lytic polysaccharide monooxygenases (LPMOs)

Z Forsberg, G Courtade - Essays in Biochemistry, 2023 - portlandpress.com
Lytic polysaccharide monooxygenases (LPMOs) have revolutionized our understanding of
how enzymes degrade insoluble polysaccharides. Compared with the substantial …

Visible light-exposed lignin facilitates cellulose solubilization by lytic polysaccharide monooxygenases

EG Kommedal, CF Angeltveit, LJ Klau… - Nature …, 2023 - nature.com
Lytic polysaccharide monooxygenases (LPMOs) catalyze oxidative cleavage of crystalline
polysaccharides such as cellulose and are crucial for the conversion of plant biomass in …

Reductants fuel lytic polysaccharide monooxygenase activity in a pH‐dependent manner

O Golten, I Ayuso‐Fernández, KR Hall… - FEBS …, 2023 - Wiley Online Library
Polysaccharide‐degrading mono‐copper lytic polysaccharide monooxygenases (LPMOs)
are efficient peroxygenases that require electron donors (reductants) to remain in the active …

Kinetic characterization of a putatively chitin-active LPMO reveals a preference for soluble substrates and absence of monooxygenase activity

L Rieder, D Petrović, P Valjamae, VGH Eijsink… - ACS …, 2021 - ACS Publications
Enzymes known as lytic polysaccharide monooxygenases (LPMOs) are recognized as
important contributors to aerobic enzymatic degradation of recalcitrant polysaccharides such …