Harnessing human genetics and stem cells for precision cardiovascular medicine

A Caudal, MP Snyder, JC Wu - Cell Genomics, 2024 - cell.com
Human induced pluripotent stem cell (iPSC) platforms are valuable for biomedical and
pharmaceutical research by providing tissue-specific human cells that retain patients' …

Multiplexed Assays of Variant Effect and Automated Patch Clam** Improve KCNH2-LQTS Variant Classification and Cardiac Event Risk Stratification

MJ O'Neill, CA Ng, T Aizawa, L Sala, S Bains… - Circulation, 2024 - ahajournals.org
BACKGROUND: Long QT syndrome is a lethal arrhythmia syndrome, frequently caused by
rare loss-of-function variants in the potassium channel encoded by KCNH2. Variant …

The impact of genetics on the long QT syndrome: myth or reality?

PJ Schwartz, L Sala - Current Opinion in Cardiology, 2023 - journals.lww.com
It is undeniable that genetics has revolutionized our mechanistic understanding of cardiac
channelopathies. Its impact has been enormous but, curiously, the way LQTS patients are …

[PDF][PDF] Assays of Variant Effect and Automated Patch Clam** Improve KCNH2-LQTS Variant Classification and Cardiac Event Risk Stratification

MJ O'Neill, CA Ng, T Aizawa, L Sala, S Bains… - Circulation, 2024 - drive.google.com
BACKGROUND: Long QT syndrome is a lethal arrhythmia syndrome, frequently caused by
rare loss-of-function variants in the potassium channel encoded by KCNH2. Variant …

Targeting the IKs Channel PKA Phosphorylation Axis to Restore Its Function in High-Risk LQT1 Variants

L Zhong, Z Yan, D Jiang, KC Weng, Y Ouyang… - Circulation …, 2024 - ahajournals.org
BACKGROUND: The KCNQ1+ KCNE1 (IKs) potassium channel plays a crucial role in
cardiac adaptation to stress, in which β-adrenergic stimulation phosphorylates the IKs …

ParSE-seq: a calibrated multiplexed assay to facilitate the clinical classification of putative splice-altering variants

MJ O'Neill, T Yang, J Laudeman… - Nature …, 2024 - nature.com
Interpreting the clinical significance of putative splice-altering variants outside canonical
splice sites remains difficult without time-intensive experimental studies. To address this, we …

Multiplexed Assays of Variant Effect and Automated Patch-clam** Improve KCNH2-LQTS Variant Classification and Cardiac Event Risk Stratification

MJ O'Neill, CA Ng, T Aizawa, L Sala, S Bains… - medRxiv, 2024 - pmc.ncbi.nlm.nih.gov
Background: Long QT syndrome (LQTS) is a lethal arrhythmia syndrome, frequently caused
by rare loss-of-function variants in the potassium channel encoded by KCNH2. Variant …