DNA in nanochannels: theory and applications
Nanofluidic structures have over the last two decades emerged as a powerful platform for
detailed analysis of DNA on the kilobase pair length scale. When DNA is confined to a …
detailed analysis of DNA on the kilobase pair length scale. When DNA is confined to a …
Exploring DNA–protein interactions on the single DNA molecule level using nanofluidic tools
K Frykholm, LK Nyberg, F Westerlund - Integrative Biology, 2017 - academic.oup.com
DNA–protein interactions are at the core of the cellular machinery and single molecule
methods have revolutionized the possibilities to study, and our understanding of these …
methods have revolutionized the possibilities to study, and our understanding of these …
Direct identification of antibiotic resistance genes on single plasmid molecules using CRISPR/Cas9 in combination with optical DNA map**
Bacterial plasmids are extensively involved in the rapid global spread of antibiotic
resistance. We here present an assay, based on optical DNA map** of single plasmids in …
resistance. We here present an assay, based on optical DNA map** of single plasmids in …
Phosphorylated CtIP bridges DNA to promote annealing of broken ends
The early steps of DNA double-strand break (DSB) repair in human cells involve the MRE11-
RAD50-NBS1 (MRN) complex and its cofactor, phosphorylated CtIP. The roles of these …
RAD50-NBS1 (MRN) complex and its cofactor, phosphorylated CtIP. The roles of these …
The effect of the kinetoplast edge loop on network percolation
J Ragotskie, N Morrison, C Stackhouse… - Journal of Polymer …, 2024 - Wiley Online Library
We use graph theory simulations and single molecule experiments to investigate percolation
properties of kinetoplasts, the topologically linked mitochondrial DNA from trypanosome …
properties of kinetoplasts, the topologically linked mitochondrial DNA from trypanosome …
Rapid identification of intact bacterial resistance plasmids via optical map** of single DNA molecules
The rapid spread of antibiotic resistance–currently one of the greatest threats to human
health according to WHO–is to a large extent enabled by plasmid-mediated horizontal …
health according to WHO–is to a large extent enabled by plasmid-mediated horizontal …
Experimental evidence of weak excluded volume effects for nanochannel confined DNA
We present experimental demonstration that weak excluded volume effects arise in DNA
nanochannel confinement. In particular, by performing measurements of the variance in …
nanochannel confinement. In particular, by performing measurements of the variance in …
[HTML][HTML] Xrs2/NBS1 promote end-bridging activity of the MRE11-RAD50 complex
C Möller, R Sharma, R Öz, G Reginato… - Biochemical and …, 2024 - Elsevier
DNA double strand breaks (DSBs) can be detrimental to the cell and need to be efficiently
repaired. A first step in DSB repair is to bring the free ends in close proximity to enable …
repaired. A first step in DSB repair is to bring the free ends in close proximity to enable …
Rapid tracing of resistance plasmids in a nosocomial outbreak using optical DNA map**
V Muller, N Karami, LK Nyberg, C Pichler… - ACS infectious …, 2016 - ACS Publications
Resistance to life-saving antibiotics increases rapidly worldwide, and multiresistant bacteria
have become a global threat to human health. Presently, the most serious threat is the …
have become a global threat to human health. Presently, the most serious threat is the …
Optical DNA map** combined with Cas9-targeted resistance gene identification for rapid tracking of resistance plasmids in a neonatal intensive care unit outbreak
The global spread of antibiotic resistance among Enterobacteriaceae is largely due to
multidrug resistance plasmids that can transfer between different bacterial strains and …
multidrug resistance plasmids that can transfer between different bacterial strains and …