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Bacterial cell wall quality control during environmental stress
Single-celled organisms must adapt their physiology to persist and propagate across a wide
range of environmental conditions. The growth and division of bacterial cells depend on …
range of environmental conditions. The growth and division of bacterial cells depend on …
Randomly barcoded transposon mutant libraries for gut commensals II: Applying libraries for functional genetics
The critical role of the intestinal microbiota in human health and disease is well recognized.
Nevertheless, there are still large gaps in our understanding of the functions and …
Nevertheless, there are still large gaps in our understanding of the functions and …
Coordination of peptidoglycan synthesis and outer membrane constriction during Escherichia coli cell division
To maintain cellular structure and integrity during division, Gram-negative bacteria must
carefully coordinate constriction of a tripartite cell envelope of inner membrane …
carefully coordinate constriction of a tripartite cell envelope of inner membrane …
ARTIST: high-resolution genome-wide assessment of fitness using transposon-insertion sequencing
Transposon-insertion sequencing (TIS) is a powerful approach for deciphering genetic
requirements for bacterial growth in different conditions, as it enables simultaneous genome …
requirements for bacterial growth in different conditions, as it enables simultaneous genome …
Outer membrane lipoprotein NlpI scaffolds peptidoglycan hydrolases within multi‐enzyme complexes in Escherichia coli
The peptidoglycan (PG) sacculus provides bacteria with the mechanical strength to maintain
cell shape and resist osmotic stress. Enlargement of the mesh‐like sacculus requires the …
cell shape and resist osmotic stress. Enlargement of the mesh‐like sacculus requires the …
Phosphorylation of the peptidoglycan synthase PonA1 governs the rate of polar elongation in mycobacteria
Cell growth and division are required for the progression of bacterial infections. Most rod-
shaped bacteria grow by inserting new cell wall along their mid-section. However …
shaped bacteria grow by inserting new cell wall along their mid-section. However …
Regulation and function of class A Penicillin-binding proteins
Highlights•Class A PBPs are essential in most bacteria.•They provide a major contribution to
overall PG synthesis during growth and cell division.•Multiple protein? protein interactions …
overall PG synthesis during growth and cell division.•Multiple protein? protein interactions …
[HTML][HTML] Modulation of peptidoglycan synthesis by recycled cell wall tetrapeptides
The bacterial cell wall is made of peptidoglycan (PG), a polymer that is essential for the
maintenance of cell shape and survival. During growth, bacteria remodel their PG, releasing …
maintenance of cell shape and survival. During growth, bacteria remodel their PG, releasing …
Transposon-insertion sequencing screens unveil requirements for EHEC growth and intestinal colonization
Enterohemorrhagic Escherichia coli O157: H7 (EHEC) is an important food-borne pathogen
that colonizes the colon. Transposon-insertion sequencing (TIS) was used to identify genes …
that colonizes the colon. Transposon-insertion sequencing (TIS) was used to identify genes …
Understanding tolerance to cell wall–active antibiotics
Antibiotic tolerance—the ability of bacteria to survive for an extended time in the presence of
bactericidal antibiotics—is an understudied contributor to antibiotic treatment failure. Herein …
bactericidal antibiotics—is an understudied contributor to antibiotic treatment failure. Herein …