Best practices for single-cell analysis across modalities

L Heumos, AC Schaar, C Lance, A Litinetskaya… - Nature Reviews …, 2023 - nature.com
Recent advances in single-cell technologies have enabled high-throughput molecular
profiling of cells across modalities and locations. Single-cell transcriptomics data can now …

Connecting past and present: single-cell lineage tracing

C Chen, Y Liao, G Peng - Protein & Cell, 2022 - academic.oup.com
Central to the core principle of cell theory, depicting cells' history, state and fate is a
fundamental goal in modern biology. By leveraging clonal analysis and single-cell RNA-seq …

A time-resolved, multi-symbol molecular recorder via sequential genome editing

J Choi, W Chen, A Minkina, FM Chardon, CC Suiter… - Nature, 2022 - nature.com
DNA is naturally well suited to serve as a digital medium for in vivo molecular recording.
However, contemporary DNA-based memory devices are constrained in terms of the …

PhyloVelo enhances transcriptomic velocity field map** using monotonically expressed genes

K Wang, L Hou, X Wang, X Zhai, Z Lu, Z Zi… - Nature …, 2024 - nature.com
Single-cell RNA sequencing (scRNA-seq) is a powerful approach for studying cellular
differentiation, but accurately tracking cell fate transitions can be challenging, especially in …

Comprehensive spatiotemporal map** of single-cell lineages in develo** mouse brain by CRISPR-based barcoding

L **: A general framework for analyzing progenitor state dynamics via retrospective lineage barcoding
W Fang, CM Bell, A Sapirstein, S Asami, K Leeper… - Cell, 2022 - cell.com
Natural and induced somatic mutations that accumulate in the genome during development
record the phylogenetic relationships of cells; whether these lineage barcodes capture the …

Map** lineage-traced cells across time points with moslin

M Lange, Z Piran, M Klein, B Spanjaard, D Klein… - Genome Biology, 2024 - Springer
Simultaneous profiling of single-cell gene expression and lineage history holds enormous
potential for studying cellular decision-making. Recent computational approaches combine …

The lives of cells, recorded

A Askary, W Chen, J Choi, LY Du, MB Elowitz… - Nature Reviews …, 2024 - nature.com
A paradigm for biology is emerging in which cells can be genetically programmed to write
their histories into their own genomes. These records can subsequently be read, and the …

New tools for lineage tracing in cancer in vivo

MG Jones, D Yang, JS Weissman - Annual Review of Cancer …, 2023 - annualreviews.org
During tumor evolution, cancer cells can acquire the ability to proliferate, invade neighboring
tissues, evade the immune system, and spread systemically. Tracking this process remains …

LinRace: cell division history reconstruction of single cells using paired lineage barcode and gene expression data

X Pan, H Li, P Putta, X Zhang - Nature Communications, 2023 - nature.com
Lineage tracing technology using CRISPR/Cas9 genome editing has enabled simultaneous
readouts of gene expressions and lineage barcodes in single cells, which allows for …