The abundance of data pertaining to the metabolism of lipids in relation to mammalian
fertilization has warranted an effort to assemble a molecular membrane model for the …

The spermatozoa of most invertebrates (eg, sea urchins) and nonmammalian vertebrates
(eg, fishes and amphibians) have full capacity to fertilize eggs upon leaving the testis …

A triple‐stain technique has been developed to score normal acrosome‐reacted human
sperm in fixed smears. Live and dead sperm are first differentiated using the vital stain …

Abstract Pachytene PIWI-interacting RNAs (piRNAs), which comprise> 80% of small RNAs
in the adult mouse testis, have been proposed to bind and regulate target RNAs like …

Prior to interaction with the oocyte, spermatozoa must undergo capacitation, which involves
a series of physio-chemical transformations that occur in the female tract. As capacitation is …

Capacitation is a prerequisite for successful fertilization by mammalian spermatozoa. This
process is generally observed in vitro in defined NaHCO3-buffered media and has been …

We have developed an assay for detecting the acrosome reaction in mouse sperm using
chlortetracycline (CTC) as a fluorescent probe. Sperm known to be intact with nonreacted …

When cholesterol is added to sperm membranes before cryopreservation, higher
percentages of motile and viable cells are recovered after thawing. However, because one …

OBJECTIVE: To evaluate the localization of phospholipase Cζ (PLCζ) in non-capacitated,
capacitated, and ionophore-treated sperm. DESIGN: Phospholipase Cζ was cloned from the …

This work analysed intracellular calcium stores of boar spermatozoa subjected to 'in
vitro'capacitation (IVC) and subsequent progesterone‐induced acrosome exocytosis (IVAE) …