Water solubility and structural stability are key merits for proteins defined by the primary
sequence and 3D-conformation. Their manipulation represents important aspects of the …

Therapeutic enzymes are valuable biopharmaceuticals in various biomedical applications.
They have been successfully applied for fibrinolysis, cancer treatment, enzyme replacement …

ProThermDB is an updated version of the thermodynamic database for proteins and mutants
(ProTherm), which has∼ 31 500 data on protein stability, an increase of 84% from the …

Designing protein mutants with both high stability and activity is a critical yet challenging
task in protein engineering. Here, we introduce PRIME, a deep learning model, which can …

Protein thermodynamic stability is essential to clarify the relationships among structure,
function, and interaction. Therefore, develo** a faster and more accurate method to …

Thermally stable proteins find extensive applications in industrial production,
pharmaceutical development, and serve as a highly evolved starting point in protein …

We provide an overview of the methods that can be used for protein structure-based
evaluation of missense variants. The algorithms can be broadly divided into those that …

Membrane proteins mediate a wide spectrum of biological processes, such as signal
transduction and cell communication. Due to the arduous and costly nature inherent to the …

Protein solubility is an attractive engineering target primarily due to its relation to yields in
protein production and manufacturing. Moreover, better knowledge of the mutational effects …

The stability of membrane proteins differs from globular proteins due to the presence of
nonpolar membrane-spanning regions. Using a dataset of 929 membrane protein mutations …