Bringing structure to cell biology with cryo-electron tomography

LN Young, E Villa - Annual review of biophysics, 2023 - annualreviews.org
Recent advances in cryo-electron microscopy have marked only the beginning of the
potential of this technique. To bring structure into cell biology, the modality of cryo-electron …

Structure and function of the nuclear pore complex

S Petrovic, GW Mobbs, CJ Bley… - Cold Spring …, 2022 - cshperspectives.cshlp.org
The nucleus, a genome-containing organelle eponymous of eukaryotes, is enclosed by a
double membrane continuous with the endoplasmic reticulum. The nuclear pore complex …

Structure of cytoplasmic ring of nuclear pore complex by integrative cryo-EM and AlphaFold

P Fontana, Y Dong, X Pi, AB Tong, CW Hecksel… - Science, 2022 - science.org
INTRODUCTION The nuclear pore complex (NPC) is the molecular conduit in the nuclear
membrane of eukaryotic cells that regulates import and export of biomolecules between the …

Lipid saturation controls nuclear envelope function

A Romanauska, A Köhler - Nature Cell Biology, 2023 - nature.com
The nuclear envelope (NE) is a spherical double membrane with elastic properties. How NE
shape and elasticity are regulated by lipid chemistry is unknown. Here we discover lipid acyl …

Visualizing the disordered nuclear transport machinery in situ

M Yu, M Heidari, S Mikhaleva, PS Tan, S Mingu… - Nature, 2023 - nature.com
The approximately 120 MDa mammalian nuclear pore complex (NPC) acts as a gatekeeper
for the transport between the nucleus and cytosol. The central channel of the NPC is filled …

Structure of the cytoplasmic ring of the Xenopus laevis nuclear pore complex

X Zhu, G Huang, C Zeng, X Zhan, K Liang, Q Xu… - Science, 2022 - science.org
INTRODUCTION The nuclear pore complex (NPC) resides on the nuclear envelope (NE)
and mediates nucleocytoplasmic cargo transport. As one of the largest cellular machineries …

Protein import into peroxisomes occurs through a nuclear pore–like phase

Y Gao, ML Skowyra, P Feng, TA Rapoport - Science, 2022 - science.org
Peroxisomes are ubiquitous organelles whose dysfunction causes fatal human diseases.
Most peroxisomal proteins are imported from the cytosol in a folded state by the soluble …

Docking a flexible basket onto the core of the nuclear pore complex

E Stankunas, A Köhler - Nature Cell Biology, 2024 - nature.com
The nuclear basket attaches to the nucleoplasmic side of the nuclear pore complex (NPC),
coupling transcription to mRNA quality control and export. The basket expands the …

Implications of a multiscale structure of the yeast nuclear pore complex

CW Akey, I Echeverria, C Ouch, I Nudelman, Y Shi… - Molecular Cell, 2023 - cell.com
Nuclear pore complexes (NPCs) direct the nucleocytoplasmic transport of macromolecules.
Here, we provide a composite multiscale structure of the yeast NPC, based on improved 3D …

Nuclear pore complexes mediate subtelomeric gene silencing by regulating PCNA levels on chromatin

SK Choudhry, ML Neal, S Li, AT Navare… - Journal of Cell …, 2023 - rupress.org
The nuclear pore complex (NPC) physically interacts with chromatin and regulates gene
expression. The Saccharomyces cerevisiae inner ring nucleoporin Nup170 has been …