Super-resolution fluorescence imaging by photoactivation or photoswitching of single
fluorophores and position determination (single-molecule localization microscopy, SMLM) …

Observation of molecular processes inside living cells is fundamental to a quantitative
understanding of how biological systems function. Specifically, decoding the complex …

INTRODUCTION Our textbook understanding of the nanoscale organization of the cell and
its relationship to the thousands of proteins that drive cellular metabolism comes largely from …

Far-field optical microscopy using focused light is an important tool in a number of scientific
disciplines including chemical,(bio) physical and biomedical research, particularly with …

Despite its short history, diffraction-unlimited fluorescence microscopy techniques have
already made a substantial imprint in the biological sciences. In this review, we describe …

Mammalian mitochondrial DNA (mtDNA) is packaged by mitochondrial transcription factor A
(TFAM) into mitochondrial nucleoids that are of key importance in controlling the …

Microscopy has gone hand in hand with the study of living systems since van Leeuwenhoek
observed living microorganisms and cells in 1674 using his light microscope. A spectrum of …

Abstract Extending three-dimensional (3D) single-molecule localization microscopy away
from the coverslip and into thicker specimens will greatly broaden its biological utility …

DNA molecules in mitochondria, just like those in the nucleus of eukaryotic cells, are
constantly damaged by noxious agents. Eukaryotic cells have developed efficient …

Mitochondria are highly dynamic organelles that exhibit a complex inner architecture. They
exhibit a smooth outer membrane and a highly convoluted inner membrane that forms …