Photophysical processes in single molecule organic fluorescent probes
EMS Stennett, MA Ciuba, M Levitus - Chemical Society Reviews, 2014 - pubs.rsc.org
The use of organic fluorescent probes in biochemical and biophysical applications of single
molecule spectroscopy and fluorescence microscopy techniques continues to increase. As …
molecule spectroscopy and fluorescence microscopy techniques continues to increase. As …
Methods for physical characterization of phase-separated bodies and membrane-less organelles
Membrane-less organelles are cellular structures which arise through the phenomenon of
phase separation. This process enables compartmentalization of specific sets of …
phase separation. This process enables compartmentalization of specific sets of …
Transbilayer lipid interactions mediate nanoclustering of lipid-anchored proteins
Understanding how functional lipid domains in live cell membranes are generated has
posed a challenge. Here, we show that transbilayer interactions are necessary for the …
posed a challenge. Here, we show that transbilayer interactions are necessary for the …
Practical guidelines for dual-color fluorescence cross-correlation spectroscopy
K Bacia, P Schwille - Nature protocols, 2007 - nature.com
Dual-color fluorescence cross-correlation spectroscopy (FCCS) allows for the determination
of molecular mobility and concentrations and for the quantitative analysis of molecular …
of molecular mobility and concentrations and for the quantitative analysis of molecular …
The nature of the amyloid-β monomer and the monomer-oligomer equilibrium
The monomer to oligomer transition initiates the aggregation and pathogenic transformation
of Alzheimer's Amyloid-β (Aβ) peptide. However, the monomeric state of this aggregation …
of Alzheimer's Amyloid-β (Aβ) peptide. However, the monomeric state of this aggregation …
Noncovalent cell surface engineering: incorporation of bioactive synthetic glycopolymers into cellular membranes
The controlled addition of structurally defined components to live cell membranes can
facilitate the molecular level analysis of cell surface phenomena. Here we demonstrate that …
facilitate the molecular level analysis of cell surface phenomena. Here we demonstrate that …
Development of a rhodamine–rhodanine-based fluorescent mercury sensor and its use to monitor real-time uptake and distribution of inorganic mercury in live …
We introduce a new rhodamine–rhodanine-based “turn-on” fluorescent sensor (RR1) and
describe its application for detection of mercury, including in solution, in live cells, and in a …
describe its application for detection of mercury, including in solution, in live cells, and in a …
Diffusivity of asphaltene molecules by fluorescence correlation spectroscopy
Using fluorescence correlation spectroscopy (FCS) we measure the translational diffusion
coefficient of asphaltene molecules in toluene at extremely low concentrations (0.03− 3.0 …
coefficient of asphaltene molecules in toluene at extremely low concentrations (0.03− 3.0 …
The Amyloid β Peptide (Aβ1-40) Is Thermodynamically Soluble at Physiological Concentrations
Precipitation of the 39− 43-residue amyloid β peptide (Aβ) is a crucial factor in Alzheimer's
disease (AD). In normal as well as in AD-afflicted brain, the Aβ concentration is estimated to …
disease (AD). In normal as well as in AD-afflicted brain, the Aβ concentration is estimated to …
Significant structural differences between transient amyloid‐β oligomers and less‐toxic fibrils in regions known to harbor familial alzheimer′ s mutations
Small oligomers of the amyloid β (Aβ) peptide, rather than the monomers or the fibrils, are
suspected to initiate Alzheimer′ s disease (AD). However, their low concentration and …
suspected to initiate Alzheimer′ s disease (AD). However, their low concentration and …