Measuring DNA modifications with the comet assay: a compendium of protocols

A Collins, P Møller, G Gajski, S Vodenková… - Nature protocols, 2023 - nature.com
The comet assay is a versatile method to detect nuclear DNA damage in individual
eukaryotic cells, from yeast to human. The types of damage detected encompass DNA …

Genotoxicity of nanomaterials: Advanced in vitro models and high throughput methods for human hazard assessment—A review

Y Kohl, E Rundén-Pran, E Mariussen, M Hesler… - Nanomaterials, 2020 - mdpi.com
Changes in the genetic material can lead to serious human health defects, as mutations in
somatic cells may cause cancer and can contribute to other chronic diseases. Genotoxic …

In vivo and in vitro models of hepatocellular carcinoma: current strategies for translational modeling

GR Romualdo, K Leroy, CJS Costa, GB Prata… - Cancers, 2021 - mdpi.com
Simple Summary Hepatocellular carcinoma (HCC) is a highly incident and deadly malignant
neoplasia, and only a few anti-HCC drugs are currently available. Thus, the development of …

Sex difference in liver diseases: How preclinical models help to dissect the sex-related mechanisms sustaining NAFLD and HCC

A Smiriglia, N Lorito, M Serra, A Perra, A Morandi… - Iscience, 2023 - cell.com
Only a few preclinical findings are confirmed in the clinic, posing a critical issue for clinical
development. Therefore, identifying the best preclinical models can help dissect molecular …

Through a glass, darkly? HepaRG and HepG2 cells as models of human phase I drug metabolism

LA Stanley, CR Wolf - Drug Metabolism Reviews, 2022 - Taylor & Francis
The pharmacokinetic and safety assessment of drug candidates is becoming increasingly
dependent upon in vitro models of hepatic metabolism and toxicity. Predominant among …
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NA Owiti, C Swartz, J Winters, Y Su, J Ge… - Nucleic acids …, 2020 - academic.oup.com
Genotoxicity testing is critical for predicting adverse effects of pharmaceutical, industrial, and
environmental chemicals. The alkaline comet assay is an established method for detecting …

Towards better prediction of xenobiotic genotoxicity: CometChip technology coupled with a 3D model of HepaRG human liver cells

A Barranger, L Le Hégarat - Archives of Toxicology, 2022 - Springer
Toxicology is facing a major change in the way toxicity testing is conducted by moving away
from animal experimentation towards animal-free methods. To improve the in vitro …