Mechanical regulation of cell-cycle progression and division
VK Gupta, O Chaudhuri - Trends in cell biology, 2022 - cell.com
Cell-cycle progression and division are fundamental biological processes in animal cells,
and their biochemical regulation has been extensively studied. An emerging body of work …
and their biochemical regulation has been extensively studied. An emerging body of work …
Present application and perspectives of organoid imaging technology
An organoid is a miniaturized and simplified in vitro model with a similar structure and
function to a real organ. In recent years, the use of organoids has increased explosively in …
function to a real organ. In recent years, the use of organoids has increased explosively in …
Mechanical compartmentalization of the intestinal organoid enables crypt folding and collective cell migration
Intestinal organoids capture essential features of the intestinal epithelium such as crypt
folding, cellular compartmentalization and collective movements. Each of these processes …
folding, cellular compartmentalization and collective movements. Each of these processes …
Organoid cell fate dynamics in space and time
Organoids are a major new tool to study tissue renewal. However, characterizing the
underlying differentiation dynamics remains challenging. Here, we developed TypeTracker …
underlying differentiation dynamics remains challenging. Here, we developed TypeTracker …
Long-term three-dimensional high-resolution imaging of live unlabeled small intestinal organoids via low-coherence holotomography
Organoids, which are miniature in vitro versions of organs, possess significant potential for
studying human diseases and elucidating their underlying mechanisms. Live imaging …
studying human diseases and elucidating their underlying mechanisms. Live imaging …
Engineering 3D micro-compartments for highly efficient and scale-independent expansion of human pluripotent stem cells in bioreactors
PJR Cohen, E Luquet, J Pletenka, A Leonard, E Warter… - Biomaterials, 2023 - Elsevier
Human pluripotent stem cells (hPSCs) have emerged as the most promising cellular source
for cell therapies. To overcome the scale-up limitations of classical 2D culture systems …
for cell therapies. To overcome the scale-up limitations of classical 2D culture systems …
Cellular shape reinforces niche to stem cell signaling in the small intestine
Niche-derived factors regulate tissue stem cells, but apart from the mechanosensory
pathways, the effect of niche geometry is not well understood. We used organoids and …
pathways, the effect of niche geometry is not well understood. We used organoids and …
[HTML][HTML] Tumor-initiating stem cell shapes its microenvironment into an immunosuppressive barrier and pro-tumorigenic niche
Tumor-initiating stem cells (TSCs) are critical for drug resistance and immune escape.
However, the mutual regulations between TSC and tumor microenvironment (TME) remain …
However, the mutual regulations between TSC and tumor microenvironment (TME) remain …
OrganoidTracker: Efficient cell tracking using machine learning and manual error correction
Time-lapse microscopy is routinely used to follow cells within organoids, allowing direct
study of division and differentiation patterns. There is an increasing interest in cell tracking in …
study of division and differentiation patterns. There is an increasing interest in cell tracking in …
[HTML][HTML] Automated live-cell single-molecule tracking in enteroid monolayers reveals transcription factor dynamics probing lineage-determining function
Lineage transcription factors (TFs) provide one regulatory level of differentiation crucial for
the generation and maintenance of healthy tissues. To probe TF function by measuring their …
the generation and maintenance of healthy tissues. To probe TF function by measuring their …