A fundamental challenge in biology is understanding the molecular details of protein
function. How mutations alter protein activity, regulation, and response to drugs is of critical …

TadA-derived cytosine base editors (TadCBEs) enable programmable C• G-to-T• A editing
while retaining the small size, high on-target activity, and low off-target activity of TadA …

Prion disease is caused by misfolding of the prion protein (PrP) into pathogenic self-
propagating conformations, leading to rapid-onset dementia and death. However …

Signaling pathways that drive gene expression are typically depicted as having a dozen or
so landmark phosphorylation and transcriptional events. In reality, thousands of dynamic …

Drug resistance is a principal limitation to the long-term efficacy of cancer therapies. Cancer
genome sequencing can retrospectively delineate the genetic basis of drug resistance, but …

Covalent chemistry represents an attractive strategy for expanding the ligandability of the
proteome, and chemical proteomics has revealed numerous electrophile-reactive cysteines …

Mutational scanning connects genetic variants to phenotype, enabling the interrogation of
protein functions, interactions and variant pathogenicity. However, current methodologies …

CRISPR screens have empowered the high-throughput dissection of gene functions;
however, more explicit genetic elements, such as codons of amino acids, require thorough …

Continual advancements in genomics have led to an ever-widening disparity between the
rate of discovery of genetic variants and our current understanding of their functions and …

CRISPR base editing screens enable analysis of disease-associated variants at scale;
however, variable efficiency and precision confounds the assessment of variant-induced …