Regulation of microtubule dynamics, mechanics and function through the growing tip

NB Gudimchuk, JR McIntosh - Nature reviews Molecular cell biology, 2021‏ - nature.com
Microtubule dynamics and their control are essential for the normal function and division of
all eukaryotic cells. This plethora of functions is, in large part, supported by dynamic …

The tubulin code and its role in controlling microtubule properties and functions

C Janke, MM Magiera - Nature Reviews Molecular Cell Biology, 2020‏ - nature.com
Microtubules are core components of the eukaryotic cytoskeleton with essential roles in cell
division, sha**, motility and intracellular transport. Despite their functional heterogeneity …

Separating the effects of nucleotide and EB binding on microtubule structure

R Zhang, B LaFrance, E Nogales - … of the National Academy of Sciences, 2018‏ - pnas.org
Microtubules (MTs) are polymers assembled from αβ-tubulin heterodimers that display the
hallmark behavior of dynamic instability. MT dynamics are driven by GTP hydrolysis within …

Microtubules: From understanding their dynamics to using them as potential therapeutic targets

Y Ilan - Journal of cellular physiology, 2019‏ - Wiley Online Library
Microtubules (MT) and actin microfilaments are dynamic cytoskeleton components involved
in a range of intracellular processes. MTs play a role in cell division, beating of cilia and …

Microtubule structure by cryo-EM: snapshots of dynamic instability

SW Manka, CA Moores - Essays in biochemistry, 2018‏ - portlandpress.com
The development of cryo-electron microscopy (cryo-EM) allowed microtubules to be
captured in their solution-like state, enabling decades of insight into their dynamic …

Taxol acts differently on different tubulin isotypes

YM Chew, RA Cross - Communications biology, 2023‏ - nature.com
Taxol is a small molecule effector that allosterically locks tubulin into the microtubule lattice.
We show here that taxol has different effects on different single-isotype microtubule lattices …

What could go wrong? A practical guide to single-particle cryo-EM: from biochemistry to atomic models

MA Cianfrocco, EH Kellogg - Journal of Chemical Information and …, 2020‏ - ACS Publications
Cryo-electron microscopy (cryo-EM) has enjoyed explosive recent growth due to
revolutionary advances in hardware and software, resulting in a steady stream of long …

Tubulin isotype regulation maintains asymmetric requirement for α-tubulin over β-tubulin

LC Wethekam, JK Moore - Journal of Cell Biology, 2023‏ - rupress.org
How cells regulate α-and β-tubulin to meet the demand for αβ-heterodimers and avoid
consequences of monomer imbalance is not understood. We investigate the role of gene …

Microtubule lattice plasticity

RA Cross - Current Opinion in Cell Biology, 2019‏ - Elsevier
In classical microtubule dynamic instability, the dynamics of the built polymer depend only
on the nucleotide state of its individual tubulin molecules. Recent work is overturning this …

Axonemal doublet microtubules can split into two complete singlets in human sperm flagellum tips

D Zabeo, JT Croft, JL Höög - FEBS letters, 2019‏ - Wiley Online Library
Motile flagella are crucial for human fertility and embryonic development. The distal tip of the
flagellum is where growth and intra‐flagellar transport are coordinated. In most model …