Cellular signaling networks are the foundation which determines the fate and function of
cells as they respond to various cues and stimuli. The discovery of fluorescent proteins over …

The study of protein subcellular distribution, their assembly into complexes and the set of
proteins with which they interact with is essential to our understanding of fundamental …

Protein-fragment complementation assays (PCAs) are widely used for investigating protein
interactions. However, the fragments used are structurally compromised and have not been …

We describe a bacterial two-hybrid system that allows an easy in vivo screening and
selection of functional interactions between two proteins. This genetic test is based on the …

The development of sensitive and versatile techniques to detect protein-protein interactions
in vivo is important for understanding protein functions. The previously described …

Dynamic networks of protein–protein interactions regulate numerous cellular processes and
determine the ability to respond appropriately to environmental stimuli. However, the …

Fluorescent probes are one of the cornerstones of real-time imaging of live cells and a
powerful tool for cell biologists. They provide high sensitivity and great versatility while …

Networks of protein interactions coordinate cellular functions. We describe a bimolecular
fluorescence complementation (BiFC) assay for determination of the locations of protein …

Many areas of biology and biotechnology have been revolutionized by the ability to label
proteins genetically by fusion to the Aequorea green fluorescent protein (GFP). In previous …

The specificity of intracellular signaling and developmental patterning in biological systems
relies on selective interactions between different proteins in specific cellular compartments …