l-Asparaginase has gained much attention for effectively treating acute lymphoblastic
leukemia (ALL) and mitigating carcinogenic acrylamide in fried foods. Due to high-dose …

Post-translational modifications (PTMs) of transcription factors regulate transcriptional
activity and play a key role in essentially all biological processes and generate …

The field of computational protein engineering has been transformed by recent
advancements in machine learning, artificial intelligence, and molecular modeling, enabling …

Computational enzyme design has been successfully applied to identify new alternatives to
natural enzymes for the biosynthesis of important compounds. However, the moderate …

GdmCl and NaSCN are two strong chaotropic salts commonly used in protein folding and
stability studies, but their microscopic mechanisms remain enigmatic. Here, by CD and …

Abstract κ-Carrageenase provides an attractive enzymatic approach to preparation of κ-
carrageenan oligosaccharides. Pseudoalteromonas tetraodonis κ-carrageenase is active at …

The Hofmeister series categorizes ions based on their effects on protein stability, yet the
microscopic mechanism remains a mystery. In this series, NaCl is neutral, Na2SO4 and …

Hyaluronate lyases are widely used in medicine and biochemical engineering and are also
applied as a tool enzyme to prepare oligosaccharides with various biological activities. To …

Cerebroside sulfotransferase (CST) is considered as therapeutic target for substrate
reduction therapy (SRT) for metachromatic leukodystrophy (MLD). The present study …

The thermostability of enzymes is an essential factor that performs a vital role during
practical applications. Inulin fructotransferases can efficiently convert inulin into bio …