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Electron microscopy studies of soft nanomaterials
This review highlights recent efforts on applying electron microscopy (EM) to soft (including
biological) nanomaterials. We will show how developments of both the hardware and …
biological) nanomaterials. We will show how developments of both the hardware and …
Isotropic reconstruction for electron tomography with deep learning
Cryogenic electron tomography (cryoET) allows visualization of cellular structures in situ.
However, anisotropic resolution arising from the intrinsic “missing-wedge” problem has …
However, anisotropic resolution arising from the intrinsic “missing-wedge” problem has …
Electron microscopy holdings of the Protein Data Bank: the impact of the resolution revolution, new validation tools, and implications for the future
As a discipline, structural biology has been transformed by the three-dimensional electron
microscopy (3DEM)“Resolution Revolution” made possible by convergence of robust cryo …
microscopy (3DEM)“Resolution Revolution” made possible by convergence of robust cryo …
High-throughput cryo-ET structural pattern mining by unsupervised deep iterative subtomogram clustering
Cryoelectron tomography directly visualizes heterogeneous macromolecular structures in
their native and complex cellular environments. However, existing computer-assisted …
their native and complex cellular environments. However, existing computer-assisted …
Deep learning improves macromolecule identification in 3D cellular cryo-electron tomograms
Cryogenic electron tomography (cryo-ET) visualizes the 3D spatial distribution of
macromolecules at nanometer resolution inside native cells. However, automated …
macromolecules at nanometer resolution inside native cells. However, automated …
Big data in contemporary electron microscopy: challenges and opportunities in data transfer, compute and management
The second decade of the twenty-first century witnessed a new challenge in the handling of
microscopy data. Big data, data deluge, large data, data compliance, data analytics, data …
microscopy data. Big data, data deluge, large data, data compliance, data analytics, data …
[HTML][HTML] Accurate size-based protein localization from cryo-ET tomograms
Cryo-electron tomography (cryo-ET) combined with sub-tomogram averaging (STA) allows
the determination of protein structures imaged within the native context of the cell at near …
the determination of protein structures imaged within the native context of the cell at near …
ScanEV–A neural network-based tool for the automated detection of extracellular vesicles in TEM images
Transmission electron microscopy (TEM) is the most widely accepted method for
visualization of extracellular vesicles (EVs), and particularly, exosomes. TEM images …
visualization of extracellular vesicles (EVs), and particularly, exosomes. TEM images …
Computational methods toward unbiased pattern mining and structure determination in cryo-electron tomography data
Cryo-electron tomography can uniquely probe the native cellular environment for
macromolecular structures. Tomograms feature complex data with densities of diverse …
macromolecular structures. Tomograms feature complex data with densities of diverse …
VP-Detector: A 3D multi-scale dense convolutional neural network for macromolecule localization and classification in cryo-electron tomograms
Background and objective: Cryo-electron tomography (cryo-ET) with subtomogram
averaging (STA) is indispensable when studying macromolecule structures and functions in …
averaging (STA) is indispensable when studying macromolecule structures and functions in …