FRET as a biomolecular research tool—understanding its potential while avoiding pitfalls

WR Algar, N Hildebrandt, SS Vogel, IL Medintz - Nature methods, 2019 - nature.com
The applications of Förster resonance energy transfer (FRET) grow with each year.
However, different FRET techniques are not applied consistently, nor are results uniformly …

Toward smart sensing by MXene

Y Li, S Huang, S Peng, H Jia, J Pang, B Ibarlucea… - Small, 2023 - Wiley Online Library
Abstract The Internet of Things era has promoted enormous research on sensors,
communications, data fusion, and actuators. Among them, sensors are a prerequisite for …

Reliability and accuracy of single-molecule FRET studies for characterization of structural dynamics and distances in proteins

G Agam, C Gebhardt, M Popara, R Mächtel, J Folz… - Nature …, 2023 - nature.com
Single-molecule Förster-resonance energy transfer (smFRET) experiments allow the study
of biomolecular structure and dynamics in vitro and in vivo. We performed an international …

In-cell structural biology by NMR: the benefits of the atomic scale

FX Theillet - Chemical reviews, 2022 - ACS Publications
In-cell structural biology aims at extracting structural information about proteins or nucleic
acids in their native, cellular environment. This emerging field holds great promise and is …

Resonance energy transfer: from fundamental theory to recent applications

GA Jones, DS Bradshaw - Frontiers in Physics, 2019 - frontiersin.org
Resonance energy transfer (RET), the transport of electronic energy from one atom or
molecule to another, has significant importance to a number of diverse areas of science …

Rapidly determining the 3D structure of proteins by surface-enhanced Raman spectroscopy

H Ma, S Yan, X Lu, YF Bao, J Liu, L Liao, K Dai… - Science …, 2023 - science.org
Despite great advances in protein structure analysis, label-free and ultrasensitive methods
to obtain the natural and dynamic three-dimensional (3D) structures are still urgently …

The emerging landscape of single-molecule protein sequencing technologies

JA Alfaro, P Bohländer, M Dai, M Filius, CJ Howard… - Nature …, 2021 - nature.com
Single-cell profiling methods have had a profound impact on the understanding of cellular
heterogeneity. While genomes and transcriptomes can be explored at the single-cell level …

[HTML][HTML] Visualizing and trap** transient oligomers in amyloid assembly pathways

EE Cawood, TK Karamanos, AJ Wilson… - Biophysical chemistry, 2021 - Elsevier
Oligomers which form during amyloid fibril assembly are considered to be key contributors
towards amyloid disease. However, understanding how such intermediates form, their …

A blind benchmark of analysis tools to infer kinetic rate constants from single-molecule FRET trajectories

M Götz, A Barth, SSR Bohr, R Börner, J Chen… - Nature …, 2022 - nature.com
Single-molecule FRET (smFRET) is a versatile technique to study the dynamics and function
of biomolecules since it makes nanoscale movements detectable as fluorescence signals …