mNGS in clinical microbiology laboratories: on the road to maturity

D Han, Z Li, R Li, P Tan, R Zhang… - Critical reviews in …, 2019 - Taylor & Francis
Metagenomic next-generation sequencing (mNGS) is increasingly being applied in clinical
laboratories for unbiased culture-independent diagnosis. Whether it can be a next routine …

Whole genome sequencing of Mycobacterium tuberculosis: current standards and open issues

CJ Meehan, GA Goig, TA Kohl, L Verboven… - NATURE reviews …, 2019 - nature.com
Whole genome sequencing (WGS) of Mycobacterium tuberculosis has rapidly progressed
from a research tool to a clinical application for the diagnosis and management of …

A sister lineage of the Mycobacterium tuberculosis complex discovered in the African Great Lakes region

JCS Ngabonziza, C Loiseau, M Marceau… - Nature …, 2020 - nature.com
The human-and animal-adapted lineages of the Mycobacterium tuberculosis complex
(MTBC) are thought to have expanded from a common progenitor in Africa. However, the …

A data compendium associating the genomes of 12,289 Mycobacterium tuberculosis isolates with quantitative resistance phenotypes to 13 antibiotics

CRyPTIC Consortium - PLoS biology, 2022 - journals.plos.org
The Comprehensive Resistance Prediction for Tuberculosis: an International Consortium
(CRyPTIC) presents here a data compendium of 12,289 Mycobacterium tuberculosis global …

Genome-wide association studies of global Mycobacterium tuberculosis resistance to 13 antimicrobials in 10,228 genomes identify new resistance mechanisms

CRyPTIC Consortium - PLoS biology, 2022 - journals.plos.org
The emergence of drug-resistant tuberculosis is a major global public health concern that
threatens the ability to control the disease. Whole-genome sequencing as a tool to rapidly …

HIV infection and multidrug resistant tuberculosis: a systematic review and meta-analysis

ZZ Sultana, FU Hoque, J Beyene… - BMC infectious …, 2021 - Springer
Background Multidrug-resistant tuberculosis (MDR-TB) in HIV infected individuals is a
serious threat to global efforts to combat tuberculosis. Inconsistent findings on the …

Application of next generation sequencing for diagnosis and clinical management of drug-resistant tuberculosis: updates on recent developments in the field

N Dookie, A Khan, N Padayatchi, K Naidoo - Frontiers in Microbiology, 2022 - frontiersin.org
The World Health Organization's End TB Strategy prioritizes universal access to an early
diagnosis and comprehensive drug susceptibility testing (DST) for all individuals with …

Population-level emergence of bedaquiline and clofazimine resistance-associated variants among patients with drug-resistant tuberculosis in southern Africa: a …

C Nimmo, J Millard, L van Dorp, K Brien… - The Lancet …, 2020 - thelancet.com
Background Bedaquiline and clofazimine are important drugs in the treatment of drug-
resistant tuberculosis and are commonly used across southern Africa, although drug …

Regimens to treat multidrug-resistant tuberculosis: past, present and future perspectives

E Pontali, MC Raviglione… - European Respiratory …, 2019 - Eur Respiratory Soc
Over the past few decades, treatment of multidrug-resistant (MDR)/extensively drug-resistant
(XDR) tuberculosis (TB) has been challenging because of its prolonged duration (up to 20 …

Application of targeted next-generation sequencing assay on a portable sequencing platform for culture-free detection of drug-resistant tuberculosis from clinical …

AM Cabibbe, A Spitaleri, S Battaglia… - Journal of clinical …, 2020 - Am Soc Microbiol
Targeted next-generation sequencing (tNGS) has emerged as a comprehensive alternative
to existing methods for drug susceptibility testing (DST) of Mycobacterium tuberculosis from …