High-throughput screening by droplet microfluidics: perspective into key challenges and future prospects

EM Payne, DA Holland-Moritz, S Sun, RT Kennedy - Lab on a Chip, 2020‏ - pubs.rsc.org
In two decades of development, impressive strides have been made for automating basic
laboratory operations in droplet-based microfluidics, allowing the emergence of a new form …

Nonlinear decision-making with enzymatic neural networks

S Okumura, G Gines, N Lobato-Dauzier, A Baccouche… - Nature, 2022‏ - nature.com
Artificial neural networks have revolutionized electronic computing. Similarly, molecular
networks with neuromorphic architectures may enable molecular decision-making on a level …

Cell-based drug screening on microfluidics

J Zhai, S Yi, Y Jia, PI Mak, RP Martins - TrAC Trends in Analytical …, 2019‏ - Elsevier
With the inherent merits of a low reagent consumption, the small amount of sample needed
and easy integration, a wide variety of microfluidic platforms (continuous-flow, droplet, digital …

Microfluidic droplet generation based on non-embedded co-flow-focusing using 3D printed nozzle

A Dewandre, J Rivero-Rodriguez, Y Vitry, B Sobac… - Scientific reports, 2020‏ - nature.com
Most commercial microfluidic droplet generators rely on the planar flow-focusing
configuration implemented in polymer or glass chips. The planar geometry, however, suffers …

Droplet Encoding‐Pairing Enabled Multiplexed Digital Loop‐Mediated Isothermal Amplification for Simultaneous Quantitative Detection of Multiple Pathogens

D Cai, Y Wang, J Zou, Z Li, E Huang… - Advanced …, 2023‏ - Wiley Online Library
Despite the advantages of digital nucleic acid analysis (DNAA) in terms of sensitivity,
precision, and resolution, current DNAA methods commonly suffer a limitation in …

Tunable encapsulation of sessile droplets with solid and liquid shells

R Lathia, S Nagpal, CD Modak, S Mishra… - nature …, 2023‏ - nature.com
Droplet encapsulations using liquid or solid shells are of significant interest in microreactors,
drug delivery, crystallization, and cell growth applications. Despite progress in droplet …

Coding of experimental conditions in microfluidic droplet assays using colored beads and machine learning supported image analysis

CM Svensson, O Shvydkiv, S Dietrich, L Mahler… - Small, 2019‏ - Wiley Online Library
To efficiently exploit the potential of several millions of droplets that can be considered as
individual bioreactors in microfluidic experiments, methods to encode different experimental …

[کتاب][B] Visions of DNA Nanotechnology at 40 for the next 40: A Tribute to Nadrian C. Seeman

N Jonoska, E Winfree - 2023‏ - library.oapen.org
This open access book provides a unique and state-of-the-art view on DNA nanotechnology
with an eye toward future developments. Intended as a tribute to Nadrian C. Seeman, who …

Nanoliter quantitative high-throughput screening with large-scale tunable gradients based on a microfluidic droplet robot under unilateral dispersion mode

Y Wei, Y Zhu, Q Fang - Analytical chemistry, 2019‏ - ACS Publications
Performing quantitative high throughput screening (qHTS) is in urgent need in current
chemical, biological, and medical research. In this work, we developed an automated …

A robust and tunable mitotic oscillator in artificial cells

Y Guan, Z Li, S Wang, PM Barnes, X Liu, H Xu, M **… - Elife, 2018‏ - elifesciences.org
Single-cell analysis is pivotal to deciphering complex phenomena like heterogeneity,
bistability, and asynchronous oscillations, where a population ensemble cannot represent …