Chemical modification of lipases: A powerful tool for activity improvement

J Noro, A Cavaco‐Paulo, C Silva - Biotechnology Journal, 2022 - Wiley Online Library
The demand for adequate and ecologically acceptable procedures to produce the most
differentiated products has been growing in recent decades, with enzymes being excellent …

Ionic Liquid‐Mediated Dynamic Polymerization for Facile Aqueous‐Phase Synthesis of Enzyme‐Covalent Organic Framework Biocatalysts

R Gao, X Kou, L Tong, ZW Li, Y Shen… - Angewandte Chemie …, 2024 - Wiley Online Library
Utilizing covalent organic framework (COF) as a hypotoxic and porous scaffold to
encapsulate enzyme (enzyme@ COF) has inspired numerous interests at the intersection of …

[HTML][HTML] Comparative characterization of baking lipase substrate specificities using emulsions and the p-nitrophenyl assay

CD Stemler, KA Scherf - LWT, 2022 - Elsevier
For use in cakes, lipases with suitable substrate specificity are needed to minimize the
release of short-chain fatty acids that cause undesirable off-flavors. We analyzed the …

Sustainable Immobilization of β-Glucosidase onto Silver Ions and AgNPs-Loaded Acrylic Fabric with Enhanced Stability and Reusability

YQ Almulaiky, J Alkabli, RM El-Shishtawy - Polymers, 2023 - mdpi.com
Modified polymer design has attracted significant attention for enzyme immobilization,
offering promising applications. In this study, amine-terminated polymers were synthesized …

Deep Eutectic Solvents as Suitable Solvents for Lipase‐Catalyzed Transesterification Reactions

J Noro, J Cabo, DS Freitas, CS Roque… - …, 2023 - Wiley Online Library
In this work, three deep eutectic mixtures (DES 1: choline chloride/urea; DES 2: choline
chloride/glycerol; and DES 3: tetrabutylammonium bromide/imidazole) were investigated as …

Preparation and characterization of a novel thermostable lipase from Thermomicrobium roseum

Y Fang, Y Zhou, Y **n, Y Shi, Z Guo, Y Li… - Catalysis Science & …, 2021 - pubs.rsc.org
In this study, a hypothetical lipase gene from Thermomicrobium roseum DSM 5159
(GenBank: ACM04789. 1) was recombinantly expressed and characterized. The TrLIP gene …

Lipase‐Catalysed Polymerization of Eutectic Mixtures

DS Freitas, V Quesado, D Rocha, J Noro… - …, 2023 - Wiley Online Library
Aiming to reduce the toxicity and operational costs often associated to chemical processes,
the enzymatic synthesis is applied herein as a sustainable route for producing polyesters …

Solvent‐free synthesis of hydrophobic and amphiphilic esters using a chemically modified lipase from Thermomyces lanuginosus: a comparative study with native …

CS Roque, M de Castro, TG Castro, C Silva… - …, 2024 - Wiley Online Library
Using lipases to catalyze the synthesis of the most differentiated type of compounds remains
one of the major challenges among scientists. Seeking more economic and advantageous …

Chemoenzymatic enantioselective and stereo-convergent syntheses of lisofylline enantiomers via lipase-catalyzed kinetic resolution and optical inversion approach

P Borowiecki, B Zdun, M Dranka - Molecular Catalysis, 2021 - Elsevier
Highly enantioselective enzymatic kinetic resolution (EKR) of racemic lisofylline is presented
for the first time. A comprehensive optimization of the key parameters of lipase-catalyzed …

Chemical modification for improving catalytic performance of lipase B from Candida antarctica with hydrophobic proline ionic liquid

X Zhang, Y Xue, Z Lu, H Xu, Y Hu - Bioprocess and Biosystems …, 2022 - Springer
In this study, a series of proline ionic liquids with different lengths of hydrophobic alkyl on the
side chain were used to modify the Candida Antarctic lipase B (CALB). The catalytic activity …