Many principles of bacterial gene regulation have been foundational to understanding
mechanisms of eukaryotic transcription. However, stark structural and functional differences …

In the past decades, advances in microscopy have made it possible to study the dynamics of
individual biomolecules in vitro and resolve intramolecular kinetics that would otherwise be …

Isogenic E. coli cells growing in a constant environment display significant variability in
growth rates, division sizes, and generation times. The guiding principle appears to be that …

How fast can a cell locate a specific chromosomal DNA sequence specified by a single
stranded oligonucleotide? To address this question we study the CRISPR-associated …

Transcription is a vital process activated by transcription factor (TF) binding. The active gene
releases a burst of transcripts before turning inactive again. While the basic course of …

The quantitative concepts used to reason about gene regulation largely derive from bacterial
studies. We show that this bacterial paradigm cannot explain the sharp expression of a …

The replisome is a multiprotein machine that carries out DNA replication. In Escherichia coli,
a single pair of replisomes is responsible for duplicating the entire 4.6 Mbp circular …

Determining whether and how a gene is transcribed are two of the central processes of life.
The conceptual basis for understanding such gene regulation arose from pioneering …

Sequence-specific binding of proteins to DNA is essential for accessing genetic information.
We derive a model that predicts an anticorrelation between the macroscopic association and …

In vivo map** of transcription-factor binding to the transcriptional output of the regulated
gene is hindered by probabilistic promoter occupancy, the presence of multiple gene copies …