Rational design of enzyme activity and enantioselectivity

Z Song, Q Zhang, W Wu, Z Pu, H Yu - Frontiers in bioengineering and …, 2023 - frontiersin.org
The strategy of rational design to engineer enzymes is to predict the potential mutants based
on the understanding of the relationships between protein structure and function, and …

Unlocking the potential of enzyme engineering via rational computational design strategies

L Zhou, C Tao, X Shen, X Sun, J Wang, Q Yuan - Biotechnology Advances, 2024 - Elsevier
Enzymes play a pivotal role in various industries by enabling efficient, eco-friendly, and
sustainable chemical processes. However, the low turnover rates and poor substrate …

Resha** substrate-binding pocket of leucine dehydrogenase for bidirectionally accessing structurally diverse substrates

T Wu, Y Wang, N Zhang, D Yin, Y Xu, Y Nie, X Mu - ACS Catalysis, 2022 - ACS Publications
Steric hindrance modification-based pocket resha** is an effective approach for enzyme–
substrate acceptance evolution. However, previous studies were limited to using the …

Classification and functional origins of stereocomplementary alcohol dehydrogenases for asymmetric synthesis of chiral secondary alcohols: a review

L Zhang, Z Sun, G Xu, Y Ni - International Journal of Biological …, 2024 - Elsevier
Alcohol dehydrogenases (ADHs) mediated biocatalytic asymmetric reduction of ketones
have been widely applied in the synthesis of optically active secondary alcohols with highly …
Q Yan, X Zhang, Y Chen, B Guo, P Zhou, B Chen… - ACS …, 2022 - ACS Publications
Glucose dehydrogenase (GDH) generally functions as an expensive cofactor NAD (P) H
regenerator in an enzyme-coupled cofactor regeneration system, as in the production of fine …

Ene-reductase-catalyzed enantioselective desymmetrization of cyclohexadienones: straightforward access to all-carbon quaternary stereocenters

X Wu, L Yang, Z Liu, K Zhang, Z Huang, F Chen - ACS Catalysis, 2024 - ACS Publications
An unprecedented enzyme-catalyzed enantioselective desymmetrization of achiral 2, 5-
cyclohexadienones has been reported. Using ene-reductases as the biocatalysts, a variety …

Batch and continuous flow asymmetric synthesis of anabolic-androgenic steroids via a single-cell biocatalytic Δ 1-dehydrogenation and C17β-carbonyl reduction …

Y Zhang, M Liu, Z Yang, J Lin, Z Huang, F Chen - Green Chemistry, 2023 - pubs.rsc.org
Chemoenzymatic asymmetric synthesis of an anabolic-androgenic steroid (+)-boldenone (3)
and its prodrug (+)-boldenone undecylenate (4) was accomplished starting from …