How to measure and evaluate binding affinities

I Jarmoskaite, I AlSadhan, PP Vaidyanathan… - Elife, 2020 - elifesciences.org
Quantitative measurements of biomolecule associations are central to biological
understanding and are needed to build and test predictive and mechanistic models. Given …

A new lens for RNA localization: liquid-liquid phase separation

EM Langdon, AS Gladfelter - Annual Review of Microbiology, 2018 - annualreviews.org
RNA localization mechanisms have been intensively studied and include localized
protection of mRNA from degradation, diffusion-coupled local entrapment of mRNA, and …

let-7 microRNAs: Their Role in Cerebral and Cardiovascular Diseases, Inflammation, Cancer, and Their Regulation

DL Bernstein, X Jiang, S Rom - Biomedicines, 2021 - mdpi.com
The let-7 family is among the first microRNAs found. Recent investigations have indicated
that it is highly expressed in many systems, including cerebral and cardiovascular systems …

hnRNP A1 dysfunction alters RNA splicing and drives neurodegeneration in multiple sclerosis (MS)

HE Salapa, PA Thibault, CD Libner, Y Ding… - Nature …, 2024 - nature.com
Neurodegeneration is the primary driver of disease progression in multiple sclerosis (MS)
resulting in permanent disability, creating an urgent need to discover its underlying …

Recognition of the TDP-43 nuclear localization signal by importin α1/β

SG Doll, H Meshkin, AJ Bryer, F Li, YH Ko… - Cell reports, 2022 - cell.com
Cytoplasmic mislocalization of the TAR-DNA binding protein of 43 kDa (TDP-43) leads to
large, insoluble aggregates that are a hallmark of amyotrophic lateral sclerosis and …

Phase separation of heterogeneous nuclear ribonucleoprotein A1 upon specific RNA‐binding observed by magnetic resonance

I Ritsch, E Lehmann, L Emmanouilidis… - Angewandte Chemie …, 2022 - Wiley Online Library
Interaction of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) with specific single‐
stranded RNA and its relation to liquid‐liquid phase separation (LLPS) were studied in vitro …

Nuclear RNA binding regulates TDP-43 nuclear localization and passive nuclear export

L Duan, BL Zaepfel, V Aksenova, M Dasso… - Cell reports, 2022 - cell.com
Nuclear clearance of the RNA-binding protein TDP-43 is a hallmark of neurodegeneration
and an important therapeutic target. Our current understanding of TDP-43 …

Tandem hnRNP A1 RNA recognition motifs act in concert to repress the splicing of survival motor neuron exon 7

I Beusch, P Barraud, A Moursy, A Clery, FHT Allain - Elife, 2017 - elifesciences.org
HnRNP A1 regulates many alternative splicing events by the recognition of splicing silencer
elements. Here, we provide the solution structures of its two RNA recognition motifs (RRMs) …

A quantitative and predictive model for RNA binding by human Pumilio proteins

I Jarmoskaite, SK Denny, PP Vaidyanathan… - Molecular cell, 2019 - cell.com
High-throughput methodologies have enabled routine generation of RNA target sets and
sequence motifs for RNA-binding proteins (RBPs). Nevertheless, quantitative approaches …

HnRNP A1 alters the structure of a conserved enterovirus IRES domain to stimulate viral translation

M Tolbert, CE Morgan, M Pollum… - Journal of molecular …, 2017 - Elsevier
Enteroviruses use a type I Internal Ribosome Entry Site (IRES) structure to facilitate protein
synthesis and promote genome replication. Type I IRES elements require auxiliary host …