[HTML][HTML] Stabilization of enzymes via immobilization: Multipoint covalent attachment and other stabilization strategies

RC Rodrigues, Á Berenguer-Murcia… - Biotechnology …, 2021 - Elsevier
The use of enzymes in industrial processes requires the improvement of their features in
many instances. Enzyme immobilization, a requirement to facilitate the recovery and reuse …

An overview of technologies for immobilization of enzymes and surface analysis techniques for immobilized enzymes

NR Mohamad, NHC Marzuki, NA Buang… - Biotechnology & …, 2015 - Taylor & Francis
The current demands of sustainable green methodologies have increased the use of
enzymatic technology in industrial processes. Employment of enzyme as biocatalysts offers …

Improvement of enzyme activity, stability and selectivity via immobilization techniques

C Mateo, JM Palomo, G Fernandez-Lorente… - Enzyme and microbial …, 2007 - Elsevier
In spite of their excellent catalytic properties, enzyme properties have to be usually improved
before their implementation at industrial scale (where many cycles of high yield processes …

Use of Alcalase in the production of bioactive peptides: A review

VG Tacias-Pascacio, R Morellon-Sterling… - International journal of …, 2020 - Elsevier
This review aims to cover the uses of the commercially available protease Alcalase in the
production of biologically active peptides since 2010. Immobilization of Alcalase has also …

Importance of the support properties for immobilization or purification of enzymes

JCS Santos, O Barbosa, C Ortiz… - …, 2015 - Wiley Online Library
Immobilization and purification of enzymes are usual requirements for their industrial use.
Both purification and immobilization have a common factor: they use a solid activated …

On the taught new tricks of enzymes immobilization: An all-inclusive overview

RA Wahab, N Elias, F Abdullah, SK Ghoshal - Reactive and Functional …, 2020 - Elsevier
The primary means of immobilizing enzymes are to boost the enzyme productivity and
operational stability, alongside facilitating the reuse of enzymes. Notwithstanding the …

Immobilization of enzymes: a literature survey

B Brena, P González-Pombo, F Batista-Viera - Immobilization of Enzymes …, 2013 - Springer
The term immobilized enzymes refers to “enzymes physically confined or localized in a
certain defined region of space with retention of their catalytic activities, and which can be …

Glyoxyl agarose: a fully inert and hydrophilic support for immobilization and high stabilization of proteins

C Mateo, JM Palomo, M Fuentes, L Betancor… - Enzyme and Microbial …, 2006 - Elsevier
Glyoxyl agarose is constituted by quite thick agarose fibres containing a large number of
very stable aldehyde groups attached to the support by very short spacer arms. Under …

Increase in conformational stability of enzymes immobilized on epoxy-activated supports by favoring additional multipoint covalent attachment☆

C Mateo, O Abian, R Fernandez–Lafuente… - Enzyme and Microbial …, 2000 - Elsevier
Epoxy supports (Eupergit C) may be very suitable to achieve the multipoint covalent
attachment of proteins and enzymes, therefore, to stabilize their three-dimensional structure …

Epoxy sepabeads: a novel epoxy support for stabilization of industrial enzymes via very intense multipoint covalent attachment

C Mateo, O Abian, G Fernández‐Lorente… - Biotechnology …, 2002 - Wiley Online Library
Sepabeads‐EP (a new epoxy support) has been utilized to immobilize‐stabilize the enzyme
penicillin G acylase (PGA) via multipoint covalent attachment. These supports are very …