Rapid, point‐of‐care antigen tests for diagnosis of SARS‐CoV‐2 infection

J Dinnes, P Sharma, S Berhane… - Cochrane database …, 2022 - cochranelibrary.com
Background Accurate rapid diagnostic tests for SARS‐CoV‐2 infection would be a useful
tool to help manage the COVID‐19 pandemic. Testing strategies that use rapid antigen tests …

[HTML][HTML] Review of COVID-19 testing and diagnostic methods

O Filchakova, D Dossym, A Ilyas, T Kuanysheva… - Talanta, 2022 - Elsevier
More than six billion tests for COVID-19 has been already performed in the world. The
testing for SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus-2) virus and …

Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA

M Patchsung, K Jantarug, A Pattama… - Nature biomedical …, 2020 - nature.com
Nucleic acid detection by isothermal amplification and the collateral cleavage of reporter
molecules by CRISPR-associated enzymes is a promising alternative to quantitative PCR …

Fast and sensitive detection of SARS-CoV-2 RNA using suboptimal protospacer adjacent motifs for Cas12a

S Lu, X Tong, Y Han, K Zhang, Y Zhang… - Nature biomedical …, 2022 - nature.com
CRISPR-based assays for the detection of nucleic acids are highly specific, yet they are not
fast, sensitive or easy to use. Here we report a one-step fluorescence assay for the detection …

Streamlined inactivation, amplification, and Cas13-based detection of SARS-CoV-2

J Arizti-Sanz, CA Freije, AC Stanton, BA Petros… - Nature …, 2020 - nature.com
The COVID-19 pandemic has highlighted that new diagnostic technologies are essential for
controlling disease transmission. Here, we develop SHINE (Streamlined Highlighting of …

Simplified Cas13-based assays for the fast identification of SARS-CoV-2 and its variants

J Arizti-Sanz, AD Bradley, YB Zhang… - Nature biomedical …, 2022 - nature.com
The widespread transmission and evolution of severe acute respiratory syndrome
coronavirus 2 (SARS-CoV-2) call for rapid nucleic acid diagnostics that are easy to use …

Photocontrolled crRNA activation enables robust CRISPR-Cas12a diagnostics

M Hu, Z Qiu, Z Bi, T Tian, Y Jiang… - Proceedings of the …, 2022 - National Acad Sciences
CRISPR diagnostics based on nucleic acid amplification faces barriers to its commercial
use, such as contamination risks and insufficient sensitivity. Here, we propose a robust …

Advanced point‐of‐care testing technologies for human acute respiratory virus detection

Z Zhang, P Ma, R Ahmed, J Wang, D Akin… - Advanced …, 2022 - Wiley Online Library
The ever‐growing global threats to human life caused by the human acute respiratory virus
(RV) infections have cost billions of lives, created a significant economic burden, and …

Detection of SARS-CoV-2 by CRISPR/Cas12a-enhanced colorimetry

Y Jiang, M Hu, AA Liu, Y Lin, L Liu, B Yu, X Zhou… - ACS …, 2021 - ACS Publications
The outbreak of COVID-19 caused a worldwide public health crisis. Large-scale population
screening is an effective means to control the spread of COVID-19. Reverse transcription …

Integrating reverse transcription recombinase polymerase amplification with CRISPR technology for the one-tube assay of RNA

W Feng, H Peng, J Xu, Y Liu, K Pabbaraju… - Analytical …, 2021 - ACS Publications
CRISPR-Cas systems integrated with nucleic acid amplification techniques improve both
analytical specificity and sensitivity. We describe here issues and solutions for the …