Label‐free super‐resolution (LFSR) imaging relies on light‐scattering processes in
nanoscale objects without a need for fluorescent (FL) staining required in super‐resolved FL …

Despite its short history, diffraction-unlimited fluorescence microscopy techniques have
already made a substantial imprint in the biological sciences. In this review, we describe …

Super-resolution microscopy opened diverse new avenues of research by overcoming the
resolution limit imposed by diffraction. Exploitation of the fluorescent emission of individual …

High-throughput super-resolution (SR) imaging is attractive for rapid and high-precision
profiling in a wide range of biomedical applications. However, current SR methods require …

Photoinduced reactions play an important role in the photocycle of fluorescent proteins from
the green fluorescent protein (GFP) family. Among such processes are photoisomerization …

Fluorescent proteins (FPs) from the GFP family have become indispensable as marker tools
for imaging live cells, tissues and entire organisms. A wide variety of these proteins have …

In his 1665 treatise, Micrographia, Robert Hooke described the many observations he had
made using a microscope, including compartment-like structures in cork samples that he …

Fundamental properties of light unavoidably impose features on images collected using
fluorescence microscopes. Accounting for these features is often critical in quantitatively …

The majority of studies of the living cell rely on capturing images using fluorescence
microscopy. Unfortunately, for centuries, diffraction of light was limiting the spatial resolution …

Super-resolution fluorescence microscopy provides unprecedented insight into cellular and
subcellular structures. However, going 'beyond the diffraction barrier'comes at a price, since …