The goldilocks approach: A review of employing design of experiments in prokaryotic recombinant protein production

A Uhoraningoga, GK Kinsella, GT Henehan, BJ Ryan - Bioengineering, 2018 - mdpi.com
The production of high yields of soluble recombinant protein is one of the main objectives of
protein biotechnology. Several factors, such as expression system, vector, host, media …

Streptokinase: an efficient enzyme in cardiac medicine

MA Zia - Protein and Peptide Letters, 2020 - ingentaconnect.com
An imbalance in oxygen supply to cardiac tissues or formation of thrombus leads to
deleterious results like pulmonary embolism, coronary heart disease and acute cardiac …

Combined utilization of lipase-displaying Pichia pastoris whole-cell biocatalysts to improve biodiesel production in co-solvent media

Z **, SY Han, L Zhang, SP Zheng, Y Wang, Y Lin - Bioresource technology, 2013 - Elsevier
Lipase-displaying whole cells appear to be efficient biocatalysts because of their low
preparation costs and simple recycling procedure. The combined utilization of Candida …

Optimization of L-asparaginase production from novel Enterobacter sp., by submerged fermentation using response surface methodology

RR Erva, AN Goswami, P Suman… - Preparative …, 2017 - Taylor & Francis
The culture conditions and nutritional rations influencing the production of extra cellular
antileukemic enzyme by novel Enterobacter aerogenes KCTC2190/MTCC111 were …

[HTML][HTML] Optimization of xylanase from Pseudomonas mohnii isolated from Simlipal Biosphere Reserve, Odisha, using response surface methodology

M Paul, DP Nayak, H Thatoi - Journal of Genetic Engineering and …, 2020 - Elsevier
Background Xylanase has long been recognized as a widely used industrially important
enzyme. There are some bacterial species already reported to produce xylanase which …

[HTML][HTML] Optimizing of nutrients for high level expression of recombinant streptokinase using pET32a expression system

S Mahmoudi, H Abtahi, A Bahador, G Mosayebi… - Mædica, 2012 - ncbi.nlm.nih.gov
Streptokinase (SK) is being increasingly used in the treatment of acute myocardial infarction
and ischemic stroke. The feeding control method using substrate balance equations is vital …

[HTML][HTML] Optimization and high level production of recombinant synthetic Streptokinase in E. coli using Response Surface Methodology

M Aghaeepoor, A Akbarzadeh, F Kobarfard… - Iranian journal of …, 2019 - ncbi.nlm.nih.gov
Streptokinase (SK) is an extracellular protein comprising 414 amino acids with considerable
clinical importance as a commonly used thrombolytic agent. Due to its wide spread …

Enhanced production of streptokinase by chemical mutagenesis of streptococcus agalactiae EBL-20

A Arshad, MA Zia, M Asghar, FA Joyia - Brazilian Archives of Biology …, 2019 - SciELO Brasil
Streptokinase (SK) is an enzyme that is used for the treatment of cardiovascular diseases.
The current study focused on the enhanced production of SK by inducing mutation in …

Optimization of amorphadiene production in engineered yeast by response surface methodology

RR Baadhe, NK Mekala, S Rao Parcha… - 3 Biotech, 2014 - Springer
Isoprenoids are among the most diverse bioactive compounds synthesized by biological
systems. The superiority of these compounds has expanded their utility from pharmaceutical …

Modular development enables rapid design of media for alternative hosts

AM Biedermann, IR Gengaro… - Biotechnology and …, 2022 - Wiley Online Library
Develo** media to sustain cell growth and production is an essential and ongoing activity
in bioprocess development. Modifications to media can often address host or product …