Three-dimensional localization of single molecules for super-resolution imaging and single-particle tracking
Single-molecule super-resolution fluorescence microscopy and single-particle tracking are
two imaging modalities that illuminate the properties of cells and materials on spatial scales …
two imaging modalities that illuminate the properties of cells and materials on spatial scales …
Exploring bacterial cell biology with single-molecule tracking and super-resolution imaging
The ability to detect single molecules in live bacterial cells enables us to probe biological
events one molecule at a time and thereby gain knowledge of the activities of intracellular …
events one molecule at a time and thereby gain knowledge of the activities of intracellular …
Video-rate nanoscopy using sCMOS camera–specific single-molecule localization algorithms
F Huang, TMP Hartwich, FE Rivera-Molina, Y Lin… - Nature …, 2013 - nature.com
Newly developed scientific complementary metal-oxide semiconductor (sCMOS) cameras
have the potential to dramatically accelerate data acquisition, enlarge the field of view and …
have the potential to dramatically accelerate data acquisition, enlarge the field of view and …
High throughput 3D super-resolution microscopy reveals Caulobacter crescentus in vivo Z-ring organization
We created a high-throughput modality of photoactivated localization microscopy (PALM)
that enables automated 3D PALM imaging of hundreds of synchronized bacteria during all …
that enables automated 3D PALM imaging of hundreds of synchronized bacteria during all …
Turning single-molecule localization microscopy into a quantitative bioanalytical tool
Single-molecule localization microscopy (SMLM) generates super-resolution images by
serially detecting individual fluorescent molecules. The power of SMLM, however, goes …
serially detecting individual fluorescent molecules. The power of SMLM, however, goes …
Defining the rate-limiting processes of bacterial cytokinesis
Bacterial cytokinesis is accomplished by the essential 'divisome'machinery. The most widely
conserved divisome component, FtsZ, is a tubulin homolog that polymerizes into the 'FtsZ …
conserved divisome component, FtsZ, is a tubulin homolog that polymerizes into the 'FtsZ …
Insights into the structure, function, and dynamics of the bacterial cytokinetic FtsZ-ring
The FtsZ protein is a highly conserved bacterial tubulin homolog. In vivo, the functional form
of FtsZ is the polymeric, ring-like structure (Z-ring) assembled at the future division site …
of FtsZ is the polymeric, ring-like structure (Z-ring) assembled at the future division site …
FtsN maintains active septal cell wall synthesis by forming a processive complex with the septum-specific peptidoglycan synthases in E. coli
FtsN plays an essential role in promoting the inward synthesis of septal peptidoglycan (sPG)
by the FtsWI complex during bacterial cell division. How it achieves this role is unclear. Here …
by the FtsWI complex during bacterial cell division. How it achieves this role is unclear. Here …
Spontaneously blinking fluorophores based on nucleophilic addition/dissociation of intracellular glutathione for live-cell super-resolution imaging
A Morozumi, M Kamiya, S Uno… - Journal of the …, 2020 - ACS Publications
Single-molecule localization microscopy (SMLM) allows the reconstruction of super-
resolution images but generally requires prior intense laser irradiation and in some cases …
resolution images but generally requires prior intense laser irradiation and in some cases …
Chemical analysis of single cells
Many biological systems contain an ensemble of cells with heterogeneous chemistry;
therefore, it is important to analyze them on an individual basis in order to elucidate the role …
therefore, it is important to analyze them on an individual basis in order to elucidate the role …