Gateway® recombinational cloning: a biological operating system

F Katzen - Expert opinion on drug discovery, 2007 - Taylor & Francis
The Gateway® cloning system sets a new trend in molecular biology by addressing the
difficulties of adaptability, efficiency and compatibility of the traditional cloning approaches …

Applications of the Restriction Free (RF) cloning procedure for molecular manipulations and protein expression

T Unger, Y Jacobovitch, A Dantes, R Bernheim… - Journal of structural …, 2010 - Elsevier
Molecular manipulations, including DNA cloning and mutagenesis are basic tools used on a
routine basis in all life-science disciplines. Over the last decade new methodologies have …

XYLEM CYSTEINE PEPTIDASE 1 and its inhibitor CYSTATIN 6 regulate pattern-triggered immunity by modulating the stability of the NADPH oxidase RESPIRATORY …

Y Liu, T Gong, X Kong, J Sun, L Liu - The Plant Cell, 2024 - academic.oup.com
Plants produce a burst of reactive oxygen species (ROS) after pathogen infection to
successfully activate immune responses. During pattern-triggered immunity (PTI), ROS are …

Using X-ray crystallography to simplify and accelerate biologics drug development

ML Brader, EN Baker, MF Dunn, TM Laue… - Journal of …, 2017 - Elsevier
Every major biopharmaceutical company incorporates a protein crystallography unit that is
central to its structure-based drug discovery efforts. Yet these capabilities are rarely …

The crystal structure of staphylococcal superantigen‐like protein 11 in complex with sialyl Lewis X reveals the mechanism for cell binding and immune inhibition

MC Chung, BD Wines, H Baker… - Molecular …, 2007 - Wiley Online Library
Staphylococcus aureus is a major pathogen that produces a family of 14 staphylococcal
superantigen‐like (SSL) proteins, which are structurally similar to superantigens but do not …

Molecular characterization and expression analysis of the SPL gene family with BpSPL9 transgenic lines found to confer tolerance to abiotic stress in Betula …

K Ning, S Chen, H Huang, J Jiang, H Yuan… - Plant Cell, Tissue and …, 2017 - Springer
SQUAMOSA promoter binding protein-like (SPL) genes encode plant-specific transcription
factors and play vital roles in plant growth and development. The BpSPL gene family has not …

Structural analysis of Chi1 chitinase from Yen-Tc: the multisubunit insecticidal ABC toxin complex of Yersinia entomophaga

JN Busby, MJ Landsberg, RM Simpson… - Journal of molecular …, 2012 - Elsevier
Yersinia entomophaga MH96 is a native New Zealand soil bacterium that secretes a large
ABC-type protein toxin complex, Yen-Tc, similar to those produced by nematode-associated …

Structures of human peroxiredoxin 3 suggest self-chaperoning assembly that maintains catalytic state

NA Yewdall, H Venugopal, A Desfosses, V Abrishami… - Structure, 2016 - cell.com
Peroxiredoxins are antioxidant proteins primarily responsible for detoxification of
hydroperoxides in cells. On exposure to various cellular stresses, peroxiredoxins can …

Structural and functional characterization of a promiscuous feruloyl esterase (Est1E) from the rumen bacterium Butyrivibrio proteoclasticus

DC Goldstone, SG Villas‐Bôas, M Till… - Proteins: Structure …, 2010 - Wiley Online Library
The release of polysaccharide from the plant cell wall is a key process to release the stored
energy from plant biomass. Within the ruminant digestive system, a host of commensal …

Structure of a eukaryotic nonribosomal peptide synthetase adenylation domain that activates a large hydroxamate amino acid in siderophore biosynthesis

TV Lee, LJ Johnson, RD Johnson, A Koulman… - Journal of Biological …, 2010 - jbc.org
Nonribosomal peptide synthetases (NRPSs) are large, multidomain proteins that are
involved in the biosynthesis of an array of secondary metabolites. We report the structure of …