Cryogenic electron microscopy (cryo-EM) is now one of the most powerful and widely used
methods to determine high-resolution structures of macromolecules. A major bottleneck of …

RNAs play critical roles in most biological processes. Although the three-dimensional (3D)
structures of RNAs primarily determine their functions, it remains challenging to …

Reconstruction maps of cryo–electron microscopy (cryo-EM) exhibit distortion when the cryo-
EM dataset is incomplete, usually caused by unevenly distributed orientations. Prior efforts …

Air-water interface (AWI) interactions during cryo-electron microscopy (cryo-EM) sample
preparation cause significant sample loss, hindering structural biology research. Organisms …

Cryo-electron microscopy (cryo-EM) has become a powerful tool to resolve high-resolution
structures of biomacromolecules in solution. However, air-water interface induced preferred …

Lipoprotein lipase (LPL) hydrolyzes triglycerides from circulating lipoproteins, releasing free
fatty acids. Active LPL is needed to prevent hypertriglyceridemia, which is a risk factor for …

Phosphoinositide 3-kinases (PI3Ks) are lipid kinases essential for growth and metabolism.
Their aberrant activation is associated with many types of cancers. Here we used single …

Cryo-sample preparation is a vital step in the process of obtaining high-resolution structures
of macromolecules by using the single-particle cryo–electron microscopy (cryo-EM) method; …

One of the key goals in single-particle cryo-microscopy is to obtain a uniform distribution of
particle orientations, so that the three-dimensional structure has isotropic resolution in …

Microtubules are tubes of about 25 nm in diameter that are critically involved in a variety of
cellular functions, including motility, compartmentalization, and division. They are …