In-cell structural biology aims at extracting structural information about proteins or nucleic
acids in their native, cellular environment. This emerging field holds great promise and is …

Motions in biomolecules are critical for biochemical reactions. In cells, many biochemical
reactions are executed inside of biomolecular condensates formed by ultradynamic …

The development of AlphaFold2 marked a paradigm-shift in the structural biology
community. Herein, we assess the ability of AlphaFold2 to predict disordered regions …

The RNA-binding protein FUS (Fused in Sarcoma) mediates phase separation in
biomolecular condensates and functions in transcription by clustering with RNA polymerase …

Liquid–liquid phase separation of flexible biomolecules has been identified as a ubiquitous
phenomenon underlying the formation of membraneless organelles that harbor a multitude …

Nuclear magnetic resonance (NMR) has long been instrumental in the characterization of
intrinsically disordered proteins (IDPs) and intrinsically disordered regions (IDRs). This …

Electrostatic interactions and charge balance are important for the formation of biomolecular
condensates involving proteins and nucleic acids. However, a detailed, atomistic picture of …

Biomolecular condensation via liquid–liquid phase separation (LLPS) of intrinsically
disordered proteins/regions (IDPs/IDRs), with and without nucleic acids, has drawn …

NMR spectroscopy has been applied to cells and tissues analysis since its beginnings, as
early as 1950. We have attempted to gather here in a didactic fashion the broad diversity of …

Biomolecules can be sequestered into membrane-less compartments, referred to as
biomolecular condensates. Experimental and computational methods have helped define …