Plant microbiome (or phytomicrobiome) engineering (PME) is an anticipated untapped
alternative strategy that could be exploited for plant growth, health and productivity under …

It has been 10 years since the introduction of modern transposon-insertion sequencing (TIS)
methods, which combine genome-wide transposon mutagenesis with high-throughput …

To support an ever-increasing population, modern agriculture faces numerous challenges
that pose major threats to global food and energy security. Plant-associated microbes, with …

One-third of all protein-coding genes from bacterial genomes cannot be annotated with a
function. Here, to investigate the functions of these genes, we present genome-wide mutant …

Rhizobia are some of the best-studied plant microbiota. These oligotrophic
Alphaproteobacteria or Betaproteobacteria form symbioses with their legume hosts …

A systems-level understanding of Gram-positive bacteria is important from both an
environmental and health perspective and is most easily obtained when high-quality …

Photosynthetic organisms provide food and energy for nearly all life on Earth, yet half of their
protein-coding genes remain uncharacterized,. Characterization of these genes could be …

Improvements in DNA synthesis and sequencing have underpinned comprehensive
assessment of gene function in bacteria and eukaryotes. Genome-wide analyses require …

Biological nitrogen fixation by Rhizobium-legume symbioses represents an environmentally
friendly and inexpensive alternative to the use of chemical nitrogen fertilizers in legume …

To fully exploit the microbial genome resources, a high-throughput experimental platform is
needed to associate genes with phenotypes at the genome level. We present here a novel …