Retroviral DNA integration

P Lesbats, AN Engelman, P Cherepanov - Chemical reviews, 2016‏ - ACS Publications
The integration of a DNA copy of the viral RNA genome into host chromatin is the defining
step of retroviral replication. This enzymatic process is catalyzed by the virus-encoded …

Function of alternative splicing

S Stamm, S Ben-Ari, I Rafalska, Y Tang, Z Zhang… - Gene, 2005‏ - Elsevier
Alternative splicing is one of the most important mechanisms to generate a large number of
mRNA and protein isoforms from the surprisingly low number of human genes. Unlike …

HIV-1 integrase forms stable tetramers and associates with LEDGF/p75 protein in human cells

P Cherepanov, G Maertens, P Proost… - Journal of Biological …, 2003‏ - jbc.org
We studied human immunodeficiency virus, type 1 (HIV-1) integrase (IN) complexes derived
from nuclei of human cells stably expressing the viral protein from a synthetic gene. We …

LEDGF/p75 functions downstream from preintegration complex formation to effect gene-specific HIV-1 integration

MC Shun, NK Raghavendra… - Genes & …, 2007‏ - genesdev.cshlp.org
LEDGF/p75 directly interacts with lentiviral integrase proteins and can modulate their
enzymatic activities and chromosomal association. A novel genetic knockout model was …

LEDGF/p75 is essential for nuclear and chromosomal targeting of HIV-1 integrase in human cells

G Maertens, P Cherepanov, W Pluymers… - Journal of Biological …, 2003‏ - jbc.org
We have reported that human immunodeficiency virus type 1 (HIV-1) integrase (IN) forms a
specific nuclear complex with human lens epithelium-derived growth factor/transcription co …

LEDGF (p75) promotes DNA-end resection and homologous recombination

M Daugaard, A Baude, K Fugger, LK Povlsen… - Nature structural & …, 2012‏ - nature.com
Lens epithelium–derived growth factor p75 splice variant (LEDGF) is a chromatin-binding
protein known for its antiapoptotic activity and ability to direct human immunodeficiency virus …

Identification of an evolutionarily conserved domain in human lens epithelium-derived growth factor/transcriptional co-activator p75 (LEDGF/p75) that binds HIV-1 …

P Cherepanov, E Devroe, PA Silver… - Journal of Biological …, 2004‏ - jbc.org
Human lens epithelium-derived growth factor/transcriptional co-activator p75 (LEDGF/p75)
protein was recently identified as a binding partner for HIV-1 integrase (IN) in human cells. In …

LEDGF/p75 determines cellular trafficking of diverse lentiviral but not murine oncoretroviral integrase proteins and is a component of functional lentiviral preintegration …

M Llano, M Vanegas, O Fregoso, D Saenz… - Journal of …, 2004‏ - journals.asm.org
Human immunodeficiency virus type 1 (HIV-1), feline immunodeficiency virus (FIV), and
Moloney murine leukemia virus (MoMLV) integrases were stably expressed to determine …

The lentiviral integrase binding protein LEDGF/p75 and HIV-1 replication

A Engelman, P Cherepanov - PLoS pathogens, 2008‏ - journals.plos.org
Retroviral replication proceeds through a stable proviral DNA intermediate, and numerous
host cell factors have been implicated in its formation. In particular, recent results have …

Integrase Mutants Defective for Interaction with LEDGF/p75 Are Impairedin Chromosome Tethering and HIV-1Replication

S Emiliani, A Mousnier, K Busschots, M Maroun… - Journal of Biological …, 2005‏ - jbc.org
The insertion of a DNA copy of its RNA genome into a chromosome of the hostcell is
mediated by the viral integrase with the help of mostlyuncharacterized cellular cofactors. We …