The proliferation of microscopy methods for live-cell imaging offers many new possibilities
for users but can also be challenging to navigate. The prevailing challenge in live-cell …

In this Primer, we focus on the most recent advancements in stimulated emission depletion
(STED) microscopy, encompassing optics, computational microscopy and probes design …

By providing spatial, molecular and morphological data over time, live-cell imaging can
provide a deeper understanding of the cellular and signalling events that determine cancer …

The optical microscope has revolutionized biology since at least the 17th Century. Since
then, it has progressed from a largely observational tool to a powerful bioanalytical platform …

Live imaging is a powerful tool, enabling scientists to observe living organisms in real time.
In particular, when combined with fluorescence microscopy, live imaging allows the …

Light-sheet fluorescence microscopy has transformed our ability to visualize and
quantitatively measure biological processes rapidly and over long time periods. In this …

Single-molecule localization microscopy (SMLM) enables crucial insights into cellular
structures and processes to be revealed at the single-molecule level. However, SMLM is …

Integrin-mediated cell adhesion is essential for cell migration, mechanotransduction and
tissue integrity. In vivo, these processes are regulated by complex physicochemical signals …

Light-sheet microscopes enable rapid high-resolution imaging of biological specimens;
however, biological processes span spatiotemporal scales. Moreover, long-term phenotypes …

To comprehensively understand tissue and organism physiology and pathophysiology, it is
essential to create complete three-dimensional (3D) cellular maps. These maps require …