We detail a six-stage planar differentiation methodology for generating human pluripotent
stem cell–derived pancreatic β cells (SC-β cells) that secrete high amounts of insulin in …

Our understanding of diabetes mellitus has benefited from a combination of clinical
investigations and work in model organisms and cell lines. Organoid models for a wide …

The generation of insulin-producing pancreatic β cells from stem cells in vitro would provide
an unprecedented cell source for drug discovery and cell transplantation therapy in …

Understanding pancreas development can provide clues for better treatments of pancreatic
diseases. However, the molecular heterogeneity and developmental trajectory of the early …

There are few in vitro models of exocrine pancreas development and primary human
pancreatic adenocarcinoma (PDAC). We establish three-dimensional culture conditions to …

Transplantation of pancreatic progenitors or insulin-secreting cells derived from human
embryonic stem cells (hESCs) has been proposed as a therapy for diabetes. We describe a …

Transcription factors regulate gene expression through binding to specific enhancer
sequences. Pancreas/duodenum homeobox protein 1 (PDX1), Neurogenin-3 (NEUROG3) …

Directed differentiation of human pluripotent stem cells into functional insulin‐producing
beta‐like cells holds great promise for cell replacement therapy for patients suffering from …

Diabetes mellitus is a metabolic disorder that affects more than 460 million people
worldwide. Type 1 diabetes (T1D) is caused by autoimmune destruction of β-cells, whereas …

The ability to generate lung and airway epithelial cells from human pluripotent stem cells
(hPSCs) would have applications in regenerative medicine, modeling of lung disease, drug …