Nowadays, time-resolved spectroscopy data can be routinely and accurately collected in UV–
vis femtosecond transient absorption spectroscopy. However, the data analysis strategy and …

Seeing is not only believing; it also includes understanding. Cellular imaging with GFP in
live cells has been transformative in many research fields. Modulation of cellular regulation …

In this perspective we introduce the basic photophysics of the excited-state intramolecular
proton transfer (ESIPT) chromophores, then the state-of-the-art development of the ESIPT …

Spinach and Spinach2 are RNA aptamers that can be used for the genetic encoding of
fluorescent RNA. Spinach2 binds and activates the fluorescence of (Z)-4-(3, 5-difluoro-4 …

In commonly studied GFP chromophore analogues such as 4-(4-hydroxybenzylidene)-1, 2-
dimethyl-1H-imidazol-5 (4H)-one (PHBDI), the dominant photoinduced processes are cis …

Members of the green fluorescent protein (GFP) family form chromophores by modifications
of three internal amino acid residues. Previously, many key characteristics of chromophores …

Molecular fluorescent indicators are versatile tools for dynamic imaging of biological
systems. We now report a class of indicators that are based on the chemigenetic …

Controlling excited-state reactivity is a long-standing challenge in photochemistry, as a
desired pathway may be inaccessible or compete with other unwanted channels. An …

Housed within the 11-stranded β-barrel of the green fluorescent protein (GFP) is the
arylideneimidazolidinone (AMI) chromophore, the component responsible for fluorescence …

The green fluorescent protein (GFP) is employed extensively as a marker in biology and the
life sciences as a result of its spectacular fluorescence properties. Here, we employ …