Masters of misdirection: peptidoglycan glycosidases in bacterial growth

A Weaver, A Taguchi, T Dörr - Journal of Bacteriology, 2023‏ - journals.asm.org
The dynamic composition of the peptidoglycan cell wall has been the subject of intense
research for decades, yet how bacteria coordinate the synthesis of new peptidoglycan with …

Bacterial metabolism and susceptibility to cell wall-active antibiotics

MR Keller, T Dörr - Advances in microbial physiology, 2023‏ - Elsevier
Bacterial infections are increasingly resistant to antimicrobial therapy. Intense research
focus has thus been placed on identifying the mechanisms that bacteria use to resist killing …

Control of bacterial cell wall autolysins by peptidoglycan crosslinking mode

L Alvarez, SB Hernandez, G Torrens, AI Weaver… - Nature …, 2024‏ - nature.com
To withstand their internal turgor pressure and external threats, most bacteria have a
protective peptidoglycan (PG) cell wall. The growth of this PG polymer relies on autolysins …

Lytic transglycosylase repertoire diversity enables intrinsic antibiotic resistance and daughter cell separation in Escherichia coli under acidic stress

JE Son, SH Park, U Choi, CR Lee - Antimicrobial Agents and …, 2024‏ - journals.asm.org
Peptidoglycan (PG) is an important architectural element that imparts physical toughness
and rigidity to the bacterial envelope. It is also a dynamic structure that undergoes …

Peptidoglycan recycling mediated by an ABC transporter in the plant pathogen Agrobacterium tumefaciens

MC Gilmore, F Cava - Nature Communications, 2022‏ - nature.com
During growth and division, the bacterial cell wall peptidoglycan (PG) is remodelled,
resulting in the liberation of PG muropeptides which are typically reinternalized and …

Peptidoglycan recycling promotes outer membrane integrity and carbapenem tolerance in Acinetobacter baumannii

N Islam, MI Kazi, KN Kang, J Biboy, J Gray, F Ahmed… - Mbio, 2022‏ - journals.asm.org
ABSTRACT β-Lactam antibiotics exploit the essentiality of the bacterial cell envelope by
perturbing the peptidoglycan layer, typically resulting in rapid lysis and death. Many Gram …

Monitoring Drug–Protein Interactions in the Bacterial Periplasm by Solution Nuclear Magnetic Resonance Spectroscopy

A Razew, Q Herail, M Miyachiro… - Journal of the …, 2024‏ - ACS Publications
The rapid spread of antimicrobial resistance across bacterial pathogens poses a serious risk
to the efficacy and sustainability of available treatments. This puts pressure on research …

In vitro studies of the protein-interaction network of cell-wall lytic transglycosylase RlpA of Pseudomonas aeruginosa

LF Avila-Cobian, S De Benedetti, C Kim… - Communications …, 2022‏ - nature.com
The protein networks of cell-wall-biosynthesis assemblies are largely unknown. A key class
of enzymes in these assemblies is the lytic transglycosylases (LTs), of which eleven exist in …

A potential space-making role in cell wall biogenesis for SltB1and DacB revealed by a beta-lactamase induction phenotype in Pseudomonas aeruginosa

J Gyger, G Torrens, F Cava, TG Bernhardt, C Fumeaux - Mbio, 2024‏ - journals.asm.org
Pseudomonas aeruginosa encodes the beta-lactamase AmpC, which promotes resistance
to beta-lactam antibiotics. Expression of ampC is induced by anhydro-muropeptides (AMPs) …

Amber‐codon suppression for spatial localization and in vivo photoaffinity capture of the interactome of the Pseudomonas aeruginosa rare lipoprotein A lytic …

LF Avila‐Cobian, H Hoshino, ME Horsman… - Protein …, 2023‏ - Wiley Online Library
The 11 lytic transglycosylases of Pseudomonas aeruginosa have overlap** activities in
the turnover of the cell‐wall peptidoglycan. Rare lipoprotein A (RlpA) is distinct among the …