Saccharomyces cerevisiae-Based Molecular Tool Kit for Manipulation of Genes from Gram-Negative Bacteria

RMQ Shanks, NC Caiazza, SM Hinsa… - Applied and …, 2006 - journals.asm.org
ABSTRACT A tool kit of vectors was designed to manipulate and express genes from a wide
range of gram-negative species by using in vivo recombination. Saccharomyces cerevisiae …

A comprehensive set of plasmids for vanillate- and xylose-inducible gene expression in Caulobacter crescentus

M Thanbichler, AA Iniesta, L Shapiro - Nucleic acids research, 2007 - academic.oup.com
Caulobacter crescentus is widely used as a powerful model system for the study of
prokaryotic cell biology and development. Analysis of this organism is complicated by a …

Vectors to express foreign genes and techniques to monitor gene expression in Pseudomonads

HP Schweizer - current opinion in biotechnology, 2001 - Elsevier
Improved tools for Pseudomonas research include small, broad-host-range vectors that
allow regulated expression from the lac operon and T7 promoters whose biology is well …

Maximizing the efficiency of vanillin production by biocatalyst enhancement and process optimization

F Luziatelli, L Brunetti, AG Ficca… - Frontiers in bioengineering …, 2019 - frontiersin.org
The rising demand of bio-vanillin and the possibility to use microbial biotransformation to
produce this compound from agroindustrial byproducts are economically attractive …

Construction and Evaluation of Plasmid Vectors Optimized for Constitutive and Regulated Gene Expression in Burkholderia cepacia Complex Isolates

MD Lefebre, MA Valvano - Applied and environmental …, 2002 - journals.asm.org
Genetic studies with Burkholderia cepacia complex isolates are hampered by the limited
availability of cloning vectors and by the inherent resistance of these isolates to the most …

Replication and partitioning of the broad-host-range plasmid RK2

K Kolatka, S Kubik, M Rajewska, I Konieczny - Plasmid, 2010 - Elsevier
The broad-host-range plasmid RK2 has been a model for studying DNA metabolism in
bacteria for many years. It is used as a vector allowing genetic manipulations in numerous …

Conservation of the Pho regulon in Pseudomonas fluorescens Pf0-1

RD Monds, PD Newell, JA Schwartzman… - Applied and …, 2006 - journals.asm.org
The Pho regulon integrates the sensing of environmental inorganic phosphate (Pi)
availability with coregulation of gene expression, mediating an adaptive response to Pi …

Interaction specificity, toxicity and regulation of a paralogous set of ParE/RelE‐family toxin–antitoxin systems

A Fiebig, CM Castro Rojas… - Molecular …, 2010 - Wiley Online Library
Toxin–antitoxin (TA) gene cassettes are widely distributed across bacteria, archaea and
bacteriophage. The chromosome of the α‐proteobacterium, Caulobacter crescentus …

The virulence regulator VirB from Shigella flexneri uses a CTP-dependent switch mechanism to activate gene expression

S Jakob, W Steinchen, J Hanßmann, J Rosum… - Nature …, 2024 - nature.com
The transcriptional antisilencer VirB acts as a master regulator of virulence gene expression
in the human pathogen Shigella flexneri. It binds DNA sequences (virS) upstream of VirB …