Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …

Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the
environment and interaction of fluorescent probes. It can report on photophysical events that …

Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique offering profound
insights into a broad spectrum of biological processes such as metabolic imaging, protein …

Applying pulsed excitation together with time-gated detection improves the fluorescence on-
off contrast in continuous-wave stimulated emission depletion (CW-STED) microscopy, thus …

Ever since their introduction two decades ago, single-molecule (SM) fluorescence methods
have matured and branched out to address numerous biological questions, which were …

Optical Spectroscopy bridges a gap by providing a background on optics while focusing on
spectroscopic methodologies, tools and instrumentations. The book introduces the most …

Fluorescence microscopy and spectroscopy data hold a wealth of information on the
investigated molecules, structures, or organisms. Nowadays, the same fluorescence data set …

In Förster resonance energy transfer (FRET) experiments, the donor (D) and acceptor (A)
fluorophores are usually attached to the macromolecule of interest via long flexible linkers of …

In the recent decade, single-molecule (sm) spectroscopy has come of age and is providing
important insight into how biological molecules function. So far our view of protein function is …

Fluorescence lifetime imaging microscopy (FLIM) is increasingly used in biomedicine,
material science, chemistry, and other related research fields, because of its advantages of …