Digital histopathology by infrared spectroscopic imaging

R Bhargava - Annual Review of Analytical Chemistry, 2023 - annualreviews.org
Infrared (IR) spectroscopic imaging records spatially resolved molecular vibrational spectra,
enabling a comprehensive measurement of the chemical makeup and heterogeneity of …

Infrared spectroscopic laser scanning confocal microscopy for whole-slide chemical imaging

K Yeh, I Sharma, K Falahkheirkhah, MP Confer… - Nature …, 2023 - nature.com
Chemical imaging, especially mid-infrared spectroscopic microscopy, enables label-free
biomedical analyses while achieving expansive molecular sensitivity. However, its slow …

Laser Direct Infrared Spectroscopy: A cutting-edge approach to microplastic detection in environmental samples

PJI Chandran, S Veerasingam - Talanta, 2024 - Elsevier
Microplastic pollution has emerged as a significant global environmental concern, affecting
marine, terrestrial, and atmospheric ecosystems. As microplastic contamination continues to …

Resolution limit in infrared chemical imaging

Y Phal, L Pfister, PS Carney… - The Journal of Physical …, 2022 - ACS Publications
Chemical imaging combines the spatial specificity of optical microscopy with the spectral
selectivity of vibrational spectroscopy. Mid-infrared (IR) absorption imaging instruments are …

Label-Free Monitoring of Coculture System Dynamics: Probing Probiotic and Cancer Cell Interactions via Infrared Spectroscopic Imaging

Y Jeong, PH Hsieh, Y Phal, R Bhargava… - Analytical …, 2024 - ACS Publications
Evaluating the dynamic interaction of microorganisms and mammalian cells is challenging
due to the lack of suitable platforms for examining interspecies interactions in biologically …

Phasor Representation Approach for Rapid Exploratory Analysis of Large Infrared Spectroscopic Imaging Data Sets

SS Mukherjee, R Bhargava - Analytical Chemistry, 2023 - ACS Publications
Infrared (IR) spectroscopic imaging is potentially useful for digital histopathology as it
provides spatially resolved molecular absorption spectra, which can subsequently yield …

On the limit of detection in infrared spectroscopic imaging

L Lux, Y Phal, PH Hsieh… - Applied spectroscopy, 2022 - journals.sagepub.com
Infrared (IR) spectroscopic imaging instruments' performance can be characterized and
optimized by an analysis of their limit of detection (LOD). Here we report a systematic …

Benchtop IR Imaging of Live Cells: Monitoring the Total Mass of Biomolecules in Single Cells

YR Chang, SM Kim, YJ Lee - Analytical chemistry, 2024 - ACS Publications
Absolute quantity imaging of biomolecules on a single cell level is critical for measurement
assurance in biosciences and bioindustries. While infrared (IR) transmission microscopy is a …

Cell Phase Identification in a Three-Dimensional Engineered Tumor Model by Infrared Spectroscopic Imaging

PH Hsieh, Y Phal, KV Prasanth, R Bhargava - Analytical chemistry, 2022 - ACS Publications
Cell cycle progression plays a vital role in regulating proliferation, metabolism, and
apoptosis. Three-dimensional (3D) cell cultures have emerged as an important class of in …

Millisecond-resolved infrared spectroscopy study of polymer brush swelling dynamics

KFA Jorissen, LB Veldscholte, M Odijk… - arxiv preprint arxiv …, 2024 - arxiv.org
We present the study of millisecond-resolved polymer brush swelling dynamics using
infrared spectroscopy with a custom-built quantum cascade laser-based infrared …