Bacterial inclusion bodies: discovering their better half

U Rinas, E Garcia-Fruitós, JL Corchero… - Trends in biochemical …, 2017 - cell.com
Bacterial inclusion bodies (IBs) are functional, non-toxic amyloids occurring in recombinant
bacteria showing analogies with secretory granules of the mammalian endocrine system …

Bacterial inclusion bodies: making gold from waste

E García-Fruitós, E Vázquez, C Díez-Gil… - Trends in …, 2012 - cell.com
Many protein species produced in recombinant bacteria aggregate as insoluble protein
clusters named inclusion bodies (IBs). IBs are discarded from further processing or are …

Bacterial inclusion bodies are industrially exploitable amyloids

A De Marco, N Ferrer-Miralles… - FEMS microbiology …, 2019 - academic.oup.com
Understanding the structure, functionalities and biology of functional amyloids is an issue of
emerging interest. Inclusion bodies, namely protein clusters formed in recombinant bacteria …

Self‐assembled proteins and peptides as scaffolds for tissue regeneration

Y Loo, M Goktas, AB Tekinay, MO Guler… - Advanced …, 2015 - Wiley Online Library
Self‐assembling proteins and peptides are increasingly gaining interest for potential use as
scaffolds in tissue engineering applications. They self‐organize from basic building blocks …

Catalytically-active inclusion bodies for biotechnology—general concepts, optimization, and application

VD Jäger, R Lamm, K Küsters, G Ölçücü… - Applied microbiology …, 2020 - Springer
Bacterial inclusion bodies (IBs) have long been considered as inactive, unfolded waste
material produced by heterologous overexpression of recombinant genes. In industrial …

Enzyme engineering for in situ immobilization

FBH Rehm, S Chen, BHA Rehm - Molecules, 2016 - mdpi.com
Enzymes are used as biocatalysts in a vast range of industrial applications. Immobilization of
enzymes to solid supports or their self-assembly into insoluble particles enhances their …

Inclusion bodies: not that bad…

A Ramón, M Señorale-Pose, M Marín - Frontiers in microbiology, 2014 - frontiersin.org
The formation of inclusion bodies (IBs) constitute a frequent event during the production of
heterologous proteins in bacterial hosts. Although the mechanisms leading to their formation …

Active protein aggregates induced by terminally attached self-assembling peptide ELK16 in Escherichia coli

W Wu, L **ng, B Zhou, Z Lin - Microbial cell factories, 2011 - Springer
Background In recent years, it has been gradually realized that bacterial inclusion bodies
(IBs) could be biologically active. In particular, several proteins including green fluorescent …

Catalytically-active inclusion bodies—Carrier-free protein immobilizates for application in biotechnology and biomedicine

U Krauss, VD Jäger, M Diener, M Pohl… - Journal of biotechnology, 2017 - Elsevier
Bacterial inclusion bodies (IBs) consist of unfolded protein aggregates and represent
inactive waste products often accumulating during heterologous overexpression of …

Nanostructured bacterial materials for innovative medicines

E Rodríguez-Carmona, A Villaverde - Trends in microbiology, 2010 - cell.com
The development of innovative medicines and personalized biomedical approaches require
the identification and implementation of new biocompatible materials produced by …