Multiplexed CRISPR technologies, in which numerous gRNAs or Cas enzymes are
expressed at once, have facilitated powerful biological engineering applications, vastly …

Based on the development of automatic devices and rapid assay methods, various high-
throughput screening (HTS) strategies have been established for improving the performance …

Monoterpene indole alkaloids (MIAs) are a diverse family of complex plant secondary
metabolites with many medicinal properties, including the essential anti-cancer therapeutics …

Through advanced mechanistic modeling and the generation of large high-quality datasets,
machine learning is becoming an integral part of understanding and engineering living …

Targeted nucleases have provided researchers with the ability to manipulate virtually any
genomic sequence, enabling the facile creation of isogenic cell lines and animal models for …

Covering: 2011–2022 The low amounts of terpenoids produced in plants and the difficulty in
synthesizing these complex structures have stimulated the production of terpenoid …

With rapid progress in DNA synthesis and sequencing, strain engineering starts to be the
rate-limiting step in synthetic biology. Here, we report a gRNA-tRNA array for CRISPR-Cas9 …

Despite the extensive use of Saccharomyces cerevisiae as a platform for synthetic biology,
strain engineering remains slow and laborious. Here, we employ CRISPR/Cas9 technology …

Competitive sustainable production in industry demands new and better biocatalysts,
optimized bioprocesses and cost-effective product recovery. Our review sheds light on the …

Saccharomyces cerevisiae is an established industrial host for production of recombinant
proteins, fuels and chemicals. To enable stable integration of multiple marker‐free …