Serial femtosecond crystallography

TRM Barends, B Stauch, V Cherezov… - Nature Reviews …, 2022‏ - nature.com
With the advent of X-ray free-electron lasers (XFELs), new, high-throughput serial
crystallography techniques for macromolecular structure determination have emerged …

Using X-ray free-electron lasers for spectroscopy of molecular catalysts and metalloenzymes

U Bergmann, J Kern, RW Schoenlein, P Wernet… - Nature Reviews …, 2021‏ - nature.com
The metal centres in metalloenzymes and molecular catalysts are responsible for the
rearrangement of atoms and electrons during complex chemical reactions, and they enable …

DIALS: implementation and evaluation of a new integration package

G Winter, DG Waterman, JM Parkhurst… - Biological …, 2018‏ - journals.iucr.org
The DIALS project is a collaboration between Diamond Light Source, Lawrence Berkeley
National Laboratory and CCP4 to develop a new software suite for the analysis of …

Structure of photosystem II and substrate binding at room temperature

ID Young, M Ibrahim, R Chatterjee, S Gul, FD Fuller… - Nature, 2016‏ - nature.com
Light-induced oxidation of water by photosystem II (PS II) in plants, algae and cyanobacteria
has generated most of the dioxygen in the atmosphere. PS II, a membrane-bound multi …

[HTML][HTML] Recent results in time resolved serial femtosecond crystallography at XFELs

AM Orville - Current Opinion in Structural Biology, 2020‏ - Elsevier
Highlights•Time-resolved serial femtosecond crystallography (tr-SFX) probes structure and
function in each sample.•X-ray free electron lasers (XFELS) provide atomic and electronic …

X-ray free-electron lasers for the structure and dynamics of macromolecules

HN Chapman - Annual review of biochemistry, 2019‏ - annualreviews.org
X-ray free-electron lasers provide femtosecond-duration pulses of hard X-rays with a peak
brightness approximately one billion times greater than is available at synchrotron radiation …

Viral internal ribosomal entry sites: four classes for one goal

J Mailliot, F Martin - Wiley Interdisciplinary Reviews: RNA, 2018‏ - Wiley Online Library
To ensure efficient propagation, viruses need to rapidly produce viral proteins after cell
entrance. Since viral genomes do not encode any components of the protein biosynthesis …

Double-flow focused liquid injector for efficient serial femtosecond crystallography

D Oberthuer, J Knoška, MO Wiedorn, KR Beyerlein… - Scientific reports, 2017‏ - nature.com
Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals
across the beam of an X-ray free-electron laser over the course of an experiment. We …

Strategies for sample delivery for femtosecond crystallography

I Martiel, HM Müller-Werkmeister… - Biological …, 2019‏ - journals.iucr.org
Highly efficient data-collection methods are required for successful macromolecular
crystallography (MX) experiments at X-ray free-electron lasers (XFELs). XFEL beamtime is …

Structural insights into functional properties of the oxidized form of cytochrome c oxidase

I Ishigami, RG Sierra, Z Su, A Peck, C Wang… - Nature …, 2023‏ - nature.com
Cytochrome c oxidase (C c O) is an essential enzyme in mitochondrial and bacterial
respiration. It catalyzes the four-electron reduction of molecular oxygen to water and …