Toxoplasmosis is caused by Toxoplasma gondii, an apicomplexan parasite that is able to
infect any nucleated cell in any warm-blooded animal. Toxoplasma gondii infects around 2 …

The clustered regularly interspaced short palindromic repeats/CRISPR-associated
(CRISPR/Cas) adaptive immune system has been extensively used for gene editing …

Apicomplexan parasites cause major human disease and food insecurity. They owe their
considerable success to highly specialized cell compartments and structures. These …

Examining host–pathogen interactions in animals can capture aspects of infection that are
obscured in cell culture. Using CRISPR-based screens, we functionally profile the entire …

Secreted virulence factors of Toxoplasma to survive in immune-competent hosts have been
extensively explored by classical genetics and in vivo CRISPR screen methods, whereas …

Toxoplasma virulence depends on its ability to evade or survive the toxoplasmacidal
mechanisms induced by interferon gamma (IFNγ). While many Toxoplasma genes involved …

Macrophages play an essential role in the early immune response against Toxoplasma and
are the cell type preferentially infected by the parasite in vivo. Interferon gamma (IFNγ) elicits …

Apicomplexan parasites, such as Toxoplasma gondii, have specific adaptations that enable
invasion and exit from the host cell. Owing to the phylogenetic distance between …

Toxoplasma can reach distant organs, especially the brain, leading to a lifelong chronic
phase. However, genes involved in related in vivo processes are currently unknown. Here …

Genome-editing technologies, in particular, CRISPR systems, are widely used for targeted
regulation of gene expression and obtaining modified human and animal cell lines, plants …