Hybridoma technology a versatile method for isolation of monoclonal antibodies, its applicability across species, limitations, advancement and future perspectives
The advancements in technology and manufacturing processes have allowed the
development of new derivatives, biosimilar or advanced improved versions for approved …
development of new derivatives, biosimilar or advanced improved versions for approved …
Antibody display technologies: selecting the cream of the crop
B Valldorf, SC Hinz, G Russo, L Pekar, L Mohr… - Biological …, 2022 - degruyter.com
Antibody display technologies enable the successful isolation of antigen-specific antibodies
with therapeutic potential. The key feature that facilitates the selection of an antibody with …
with therapeutic potential. The key feature that facilitates the selection of an antibody with …
Optimization of therapeutic antibodies by predicting antigen specificity from antibody sequence via deep learning
The optimization of therapeutic antibodies is time-intensive and resource-demanding,
largely because of the low-throughput screening of full-length antibodies (approximately 1× …
largely because of the low-throughput screening of full-length antibodies (approximately 1× …
Innovative strategies of reprogramming immune system cells by targeting CRISPR/Cas9-based genome-editing tools: a new era of cancer management
The recent developments in the study of clustered regularly interspaced short palindromic
repeats/associated protein 9 (CRISPR/Cas9) system have revolutionized the art of genome …
repeats/associated protein 9 (CRISPR/Cas9) system have revolutionized the art of genome …
Generalized extracellular molecule sensor platform for programming cellular behavior
Strategies for expanding the sensor space of designer receptors are urgently needed to
tailor cell-based therapies to respond to any type of medically relevant molecules. Here, we …
tailor cell-based therapies to respond to any type of medically relevant molecules. Here, we …
In mitosis integrins reduce adhesion to extracellular matrix and strengthen adhesion to adjacent cells
M Huber, J Casares-Arias, R Fässler, DJ Müller… - Nature …, 2023 - nature.com
To enter mitosis, most adherent animal cells reduce adhesion, which is followed by cell
rounding. How mitotic cells regulate adhesion to neighboring cells and extracellular matrix …
rounding. How mitotic cells regulate adhesion to neighboring cells and extracellular matrix …
[HTML][HTML] Engineering protein-secreting plasma cells by homology-directed repair in primary human B cells
The ability to engineer primary human B cells to differentiate into long-lived plasma cells and
secrete a de novo protein may allow the creation of novel plasma cell therapies for protein …
secrete a de novo protein may allow the creation of novel plasma cell therapies for protein …
Integrating high‐throughput screening and sequencing for monoclonal antibody discovery and engineering
C Parola, D Neumeier, ST Reddy - Immunology, 2018 - Wiley Online Library
Monoclonal antibody discovery and engineering is a field that has traditionally been
dominated by high‐throughput screening platforms (eg hybridomas and surface display). In …
dominated by high‐throughput screening platforms (eg hybridomas and surface display). In …
High-throughput antibody engineering in mammalian cells by CRISPR/Cas9-mediated homology-directed mutagenesis
Antibody engineering is often performed to improve therapeutic properties by directed
evolution, usually by high-throughput screening of phage or yeast display libraries …
evolution, usually by high-throughput screening of phage or yeast display libraries …
Augmenting adaptive immunity: progress and challenges in the quantitative engineering and analysis of adaptive immune receptor repertoires
The adaptive immune system is a natural diagnostic sensor and therapeutic. It recognizes
threats earlier than clinical symptoms manifest and neutralizes antigens with exquisite …
threats earlier than clinical symptoms manifest and neutralizes antigens with exquisite …